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Ammonia oxidizing bacterium growth promoter and preparation method

A technology for growth promoters and ammonia oxidizing bacteria, applied in chemical instruments and methods, adding compounds to stimulate growth, bacteria, etc., can solve the problems of no promotion, slow growth of ammonia oxidizing bacteria, etc., to accelerate cell growth, Improve the effect of wastewater treatment and shorten the effect of enzymatic reaction process

Active Publication Date: 2016-06-01
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CN201110315549.6 discloses a short-range nitrification and denitrification granular sludge cultivation method, which is characterized in that 5-15mg / L hydroxylamine is regularly added; CN201010168453.7 discloses a method for quickly starting an anoxic ammonia oxidation biofilter, It is characterized in that hydroxylamine is added in step (2) to induce the inoculated sludge to transform into anoxic ammonium oxidation biofilm. The above invention mainly utilizes the inhibitory effect of hydroxylamine on nitrite oxidizing bacteria to promote short-term nitrification, but slow growth Ammonia oxidizing bacteria did not promote their own growth

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  • Ammonia oxidizing bacterium growth promoter and preparation method
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Embodiment Construction

[0018] The solutions of the present invention will be described in detail below in conjunction with specific embodiments.

[0019] The formulations of the ammonia oxidizing bacteria growth promoter required in Examples 1 to 4 and Comparative Examples 1 to 6 are shown in Table 1.

[0020] Formulation and ratio of accelerator of table 1

[0021]

[0022] The wastewater discharged from a factory is treated by short-range nitrification and denitrification. The concentration of ammonia nitrogen in the wastewater is about 200mg / L. After 20 days after the device was started, the concentration of ammonia nitrogen in the effluent is still as high as 80mg / L, and the nitrification rate is only 40%. Use the accelerator configured in the above table with a concentration of 0.5g / L, and add it according to the concentration of the accelerator in the sewage at 15mg / L every day. After 15 days of adding, the nitrosation rate increases to more than 60% and stop adding. , the system continued...

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Abstract

The invention discloses an ammonia oxidizing bacterium growth promoter, which consists of metal salts, polyamine substances, inorganic acid hydroxylamine and Na2SO3, wherein in parts by weight, the metal salts account for 40-100 parts by weight, preferably 50-80 parts by weight; the polyamine substances account for 5-30 parts by weight, preferably 10-20 parts by weight; the inorganic acid hydroxylamine accounts for 0.05-1.5 parts by weight, preferably 0.1-1.0 part by weight; and the Na2SO3 accounts for 10-40 parts by weight, preferably 20-30 parts by weight; the metal salts include calcium salt, ferrous salt and copper salt; and the molar ratio of Ca<2>+ to Fe<2+> to Cu<2+> is (5-15) to (1-8) to (0.5-5), and preferably (8-12) to (2-6) to (1-4). The growth promoter is simple in formula and easy to prepare; and the growth promoter can be applied to the culture process of ammonia oxidizing bacteria and can be also directly added to a sewage treatment system, so as to accelerate the starting of a partial nitrification-denitrification and partial nitrification-anaerobic ammonia oxidation process and to achieve stable running.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an ammonia oxidizing bacteria growth promoter and a preparation method. Background technique [0002] Biological denitrification is mainly accomplished by nitrifying bacteria and denitrifying bacteria. Nitrifying bacteria are chemotrophic microorganisms. Biological cells can only use the energy stored in the form of ATP, etc., and cannot directly use the free energy released by chemical reactions. In aerobic metabolism, ATP is mainly synthesized by oxidative phosphorylation of the respiratory chain. The ammonia oxidative phosphorylation efficiency is very low, and the ATP that can be produced is very limited, and the energy is mainly used for electron transition to a higher energy level, which makes the growth of nitrifying bacteria very slow, and the generation period is 8~36h. The cell wall of nitrifying bacteria has low peptidoglycan content and high protein and fat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20C02F3/34C02F3/30
Inventor 高会杰孙丹凤郭志华赵胜楠郭宏山
Owner CHINA PETROLEUM & CHEM CORP
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