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Method for determining glucose based on paper-based electrochemical device

An electrochemical and glucose technology, applied in the field of biotechnology detection, can solve the problems of fast, simple, real-time analysis, expensive instruments, high operating costs, etc. of sample analysis, and achieve improved sensitivity and reproducibility, low cost, make simple effects

Inactive Publication Date: 2016-05-25
NANTONG UNIVERSITY
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Problems solved by technology

[0003] However, these methods generally have the disadvantages of expensive instruments, high operating costs, and not easy to carry, and are greatly limited in continuous monitoring and on-site measurement, and cannot meet the requirements of fast, simple, and real-time analysis in sample analysis. Therefore, Designing a simple, accurate, fast, sensitive, and easy-to-operate glucose detection method has aroused the interest of many researchers

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  • Method for determining glucose based on paper-based electrochemical device
  • Method for determining glucose based on paper-based electrochemical device
  • Method for determining glucose based on paper-based electrochemical device

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Embodiment Construction

[0036] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0037] Such as Figure 1 to Figure 2 Shown in, a kind of method for measuring glucose based on paper-based electrochemical device comprises the steps:

[0038] (1) The detection platform uses filter paper as the paper-based substrate, and the electrodes are manually drawn with a pencil, and graphite is used as the electrode material. The detection platform includes two parts, the detection area and the enzyme area, and each part has a circular hydrophilic area. The area outside the water area is the hydrophobic area;

[0039] (2) According to the principle of glucose oxidase oxidation reaction, glucose is measured by amperometry, and the detection platform can sensitively measure glucose in the presence of interfering substances, and obtain a working curve. In the blood sample detection, firstly, the sample to be tested is centrifuged and precipitated, then...

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Abstract

The invention discloses a method for determining glucose based on a paper-based electrochemical device. The method comprises the following steps that 1, filter paper serves as a detection platform of a paper-based substrate, an electrode is manually drawn with a pencil, and graphite serves as an electrode material, wherein the detection platform comprises two areas, namely a detection area and an enzyme area, each area is provided with a round hydrophilic area, and the part except the hydrophilic area is a hydrophobic area; 2, according to the principle of glucose oxidase oxidizing reaction, ferrocenecarboxylic acid serves as an electronic mediator, glucose is determined by adopting an amperometric method, and the detection platform can sensitively determine the glucose in the presence of interferent; a sample to be detected is added to the detection platform, and the actual blood sugar concentration of the sample can be detected. The paper-based electrochemical system adopted in the method is the novel electrochemical device, the electrochemical device is simple in manufacture and low in cost and can be used in lots of application fields including portable and disposable analytical equipment for clinical diagnosis, food quality control, environment monitoring and the like.

Description

technical field [0001] The invention relates to the field of biotechnology detection, in particular to a method for measuring glucose based on a paper-based electrochemical device. Background technique [0002] Glucose (Glucose) is the most widely distributed and most important monosaccharide in nature. It is also one of the important nutrients and main sources of heat for the human body. Therefore, blood sugar must be maintained at a certain level to maintain the needs of various organs and tissues in the body; hypoglycemia It brings great harm to patients. In mild cases, it can cause memory loss, unresponsiveness, dementia, and coma. In severe cases, it can be life-threatening. High blood sugar can cause vascular disease. Abnormal blood sugar can also lead to other metabolic diseases and complications. Harmful to human health; therefore, the detection of blood sugar is of great significance for the rapid diagnosis of related diseases in clinical practice, the research of o...

Claims

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Application Information

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IPC IPC(8): G01N27/416
CPCG01N27/416
Inventor 于春梅
Owner NANTONG UNIVERSITY
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