Method for determining glucose based on paper-based electrochemical device

Abstract

The invention discloses a method for determining glucose based on a paper-based electrochemical device. The method comprises the following steps that 1, filter paper serves as a detection platform of a paper-based substrate, an electrode is manually drawn with a pencil, and graphite serves as an electrode material, wherein the detection platform comprises two areas, namely a detection area and an enzyme area, each area is provided with a round hydrophilic area, and the part except the hydrophilic area is a hydrophobic area; 2, according to the principle of glucose oxidase oxidizing reaction, ferrocenecarboxylic acid serves as an electronic mediator, glucose is determined by adopting an amperometric method, and the detection platform can sensitively determine the glucose in the presence of interferent; a sample to be detected is added to the detection platform, and the actual blood sugar concentration of the sample can be detected. The paper-based electrochemical system adopted in the method is the novel electrochemical device, the electrochemical device is simple in manufacture and low in cost and can be used in lots of application fields including portable and disposable analytical equipment for clinical diagnosis, food quality control, environment monitoring and the like.

Description

technical field [0001] The invention relates to the field of biotechnology detection, in particular to a method for measuring glucose based on a paper-based electrochemical device. Background technique [0002] Glucose (Glucose) is the most widely distributed and most important monosaccharide in nature. It is also one of the important nutrients and main sources of heat for the human body. Therefore, blood sugar must be maintained at a certain level to maintain the needs of various organs and tissues in the body; hypoglycemia It brings great harm to patients. In mild cases, it can cause memory loss, unresponsiveness, dementia, and coma. In severe cases, it can be life-threatening. High blood sugar can cause vascular disease. Abnormal blood sugar can also lead to other metabolic diseases and complications. Harmful to human health; therefore, the detection of blood sugar is of great significance for the rapid diagnosis of related diseases in clinical practice, the research of o...

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Problems solved by technology

[0003] However, these methods generally have the disadvantages of expensive instruments, high operating costs, and not easy to carry, and are greatly limited in continuous monitoring and on-site measurement, and cannot meet the requirements of fast, simple, and real-time analysis in sample analysis. Therefore, Designing a simple, accurate, fast, sensitive, and easy-to-operate glucose detection method has aroused the interest of many researchers

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