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Expiration sensor, as well as preparation method and application thereof

A sensor and substrate technology, applied in the field of breath analysis and clinical detection, can solve the problems of weak Raman excitation efficiency and limit wide application, and achieve the effect of Raman enhancement effect, good repeatability and simple process

Active Publication Date: 2016-05-25
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extremely weak Raman excitation efficiency of analytes has always limited its wider application. Therefore, how to increase the Raman signal has always been a research hotspot for researchers, and the use of surface-enhanced Raman scattering effect to greatly enhance Raman Mann signals are considered to be of great application value

Method used

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  • Expiration sensor, as well as preparation method and application thereof
  • Expiration sensor, as well as preparation method and application thereof
  • Expiration sensor, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Step 1: Sputter a 300nm gold film on the cleaned glass as a substrate. 2 microliters of 0.25 g / L graphene oxide solution was drop-coated on the cleaned substrate surface; after natural drying, the formed graphene oxide film was as follows: figure 1 As shown in A and C, the dispersion is flat.

[0054] Step 2, place it in a space of 50 cubic centimeters, and add 100ml of hydrazine hydrate solution (85%, v / v) to the surrounding area of ​​the substrate as a source of hydrazine vapor, seal it and place it in a thermostat at 37°C for 12 hours ;

[0055] Step 3, after taking it out, let it stand still for half an hour, add 1 microliter of chloroauric acid solution (5mM) dropwise, and let it dry naturally. Finally, the electrodes were carefully washed with water to obtain a breath sensor based on graphene and gold nanoparticles, preserved in N 2 Environment. The obtained sensor surface is as figure 1 As shown in B and D, the AuNPs are uniform in size and well distribute...

Embodiment 2

[0060] Step 1: A 200nm gold film is sputtered on a cleaned silicon wafer as a substrate. 2 microliters of 0.5 g / L graphene oxide solution was drip-coated on the cleaned substrate surface;

[0061] Step 2, after natural drying, place it in a space of 200 cubic centimeters, and add 1000ml hydrazine hydrate solution (85%, v / v) to the surrounding area of ​​the substrate as a source of hydrazine vapor, seal it and place it at a constant temperature of 37°C 12 hours in box.

[0062] Step 3, after taking it out, let it stand still for half an hour, add 1 microliter of chloroplatinic acid solution (10 mM) dropwise, and let it dry naturally. Finally, careful washing with water resulted in a breath sensor based on graphene and platinum nanoparticles. Yield is about 75%.

[0063]The exhalation sensor was placed in a 500ml airbag filled with exhaled air, and was allowed to stand for adsorption at 37°C for 6 hours. After taking out, use HORIBA LabRAMHREvolution spectrophotometer to det...

Embodiment 3

[0065] Step 1, sputtering a 500nm copper film on the cleaned copper sheet as a base. 2 microliters of 0.25 g / L graphene oxide solution was dropped on the surface of the substrate after cleaning;

[0066] Step 2, after natural drying, place it in a space of 50 cubic centimeters, and add 100ml hydrazine hydrate solution (85%) to the surrounding area of ​​the substrate as a source of hydrazine vapor, seal it and place it in a thermostat at 37°C for 12 hours .

[0067] Step 3, after taking it out, let it stand for half an hour, add dropwise 10 microliters of silver nitrate solution (1mM), in N 2 Dry naturally. Finally, careful washing with water resulted in a breath sensor based on graphene and silver nanoparticles. The yield is greater than 63%.

[0068] The exhalation sensor was placed in a 500ml airbag filled with exhaled air, and was allowed to stand for adsorption at 37°C for 3 hours. After taking out, use HORIBA LabRAMHREvolution spectrophotometer to detect, the conditi...

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Abstract

The invention discloses an expiration sensor, as well as a preparation method and an application thereof. The preparation method comprises the following steps: the metal film surface of a substrate which is coated with a metal film is coated with a graphene oxide solution, and is dried, and the substrate with the surface coated with a graphene oxide film is obtained; the obtained substrate is placed in hydrazine steam for reduction, and the substrate with the surface coated with a graphene film is obtained; the graphene film surface of the obtained substrate with the surface coated with the graphene film is coated with a metal ion solution, in-situ reduction is performed to fix a metal nanometer material on the surface of the graphene, drying is performed, and the expiration sensor is obtained. The expiration sensor has the advantages of outstanding surface enhanced raman scattering effect and stability, simple process in detection of stomach cancer markers in expiratory gas, low cost and reliable results; graphene not only carries metal nanoparticles, but also is adopted as an absorption layer for selectively absorbing the stomach cancer markers enriched in the expiratory gas, therefore, the expiration sensor disclosed by the invention and the application of the expiration sensor in detection of the cancer markers in the expiratory gas has a broad prospect.

Description

technical field [0001] The invention relates to the field of breath analysis and clinical detection, relates to a breath sensor and its preparation method and application, in particular to a method for diagnosing gastric cancer by detecting gastric cancer markers in breath, which can achieve early gastric cancer detection and distinguish early and advanced gastric cancer. Background technique [0002] Survival of cancer patients is greatly improved when cancer is detected and treated early. For gastric cancer, patients diagnosed with advanced disease have an approximately 10% 5-year survival rate, while patients diagnosed with early-stage disease have a 90% 5-year survival rate. However, with the current state of the art, most gastric cancer patients are usually diagnosed with advanced disease. The sooner cancer is detected, the easier it is to treat cancer. In addition, accurate gastric cancer classification is helpful to choose the correct surgical plan, and it is also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65C01B31/04
CPCG01N21/658
Inventor 崔大祥陈云生
Owner SHANGHAI JIAO TONG UNIV
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