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Reverse micelle emulsion used for biological enzyme immobilization and biological enzyme immobilization method thereof

A technology of biological enzymes and reverse micelles, applied to biochemical equipment and methods, fixed on or in inorganic carriers, fixed on/in organic carriers, etc., can solve problems such as the reduction of biological enzyme activity, and achieve Avoid reduction, avoid loss, effect of high catalytic performance

Inactive Publication Date: 2016-05-25
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved in the present invention is to overcome the problem of using nano-Fe in the prior art 3 o 4 The technical defect of reducing the activity of biological enzymes after the immobilization of biological enzymes by magnetic particles; and then providing a reverse micellar emulsion for immobilization of biological enzymes and a method for immobilizing biological enzymes

Method used

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  • Reverse micelle emulsion used for biological enzyme immobilization and biological enzyme immobilization method thereof
  • Reverse micelle emulsion used for biological enzyme immobilization and biological enzyme immobilization method thereof
  • Reverse micelle emulsion used for biological enzyme immobilization and biological enzyme immobilization method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0045] A reverse micellar emulsion for immobilizing biological enzymes, comprising the following components in parts by weight:

[0046] OP-10: 15 servings

[0047] n-octanol: 21 parts

[0048] Isooctane: 48 parts

[0049] Tris-HCl buffer: 0.5 parts

[0050] Distilled water: 3.5 parts.

[0051] This embodiment gives birth to the method for immobilizing biological enzyme, comprises the following steps:

[0052] 1), OP-10: 15 parts by weight, n-octanol: 21 parts by weight, isooctane: 48 parts by weight, Tris-HCl buffer solution: 0.5 parts by weight, distilled water: 3.5 parts by weight, mix and stir evenly to obtain a mixed Lotion;

[0053] 2), the mixed emulsion obtained in 1) is subjected to ultrasonic treatment to form a reverse micellar system; the intensity of the ultrasonic wave is 0.5KW / m 3 , the ultrasonic frequency is 18KHz, and the processing time is 2 hours;

[0054] 3), add 2 parts by weight of Candida lipase, nano Fe 3 o 4 10 parts by weight of magnetic par...

Embodiment 2

[0057] A reverse micellar emulsion for immobilizing biological enzymes, comprising the following components in parts by weight:

[0058] OP-10: 14 servings

[0059] n-octanol: 22 parts

[0060] Isooctane: 45 parts

[0061] Tris-HCl buffer: 0.8 parts

[0062] Distilled water: 3.2 parts.

[0063] This embodiment gives birth to the method for immobilizing biological enzyme, comprises the following steps:

[0064] 1), OP-10: 14 parts by weight, n-octanol: 22 parts by weight, isooctane: 45 parts by weight, Tris-HCl buffer solution: 0.8 parts by weight, distilled water: 3.2 parts by weight, mix and stir evenly to obtain a mixed Lotion;

[0065] 2), the mixed emulsion obtained in 1) is subjected to ultrasonic treatment to form a reverse micellar system; the intensity of the ultrasonic wave is 1.2KW / m 3 , the ultrasonic frequency is 40KHz, and the processing time is 0.5 hours;

[0066] 3), add 5 parts by weight of Candida lipase, nano Fe 3 o 4 10 parts by weight of magnetic p...

Embodiment 3

[0069] A reverse micellar emulsion for immobilizing biological enzymes, comprising the following components in parts by weight:

[0070] OP-10: 12 servings

[0071] n-octanol: 20 parts

[0072] Isooctane: 46 parts

[0073] Tris-HCl buffer: 0.8 parts

[0074] Distilled water: 3.2 parts.

[0075] This embodiment gives birth to the method for immobilizing biological enzyme, comprises the following steps:

[0076] 1), OP-10: 12 parts by weight, n-octanol: 20 parts by weight, isooctane: 46 parts by weight, Tris-HCl buffer solution: 0.8 parts by weight, distilled water: 3.2 parts by weight, mix and stir evenly to obtain a mixed Lotion;

[0077] 2), the mixed emulsion obtained in 1) is subjected to ultrasonic treatment to form a reverse micellar system; the intensity of the ultrasonic wave is 0.8KW / m 3 , the ultrasonic frequency is 30KHz, and the processing time is 1 hour;

[0078] 3), add 8 parts by weight of Candida lipase, nano Fe 3 o 4 10 parts by weight of magnetic part...

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Abstract

The invention discloses reverse micelle emulsion used for biological enzyme immobilization. The reverse micelle emulsion used for the biological enzyme immobilization comprises the following components in parts by weight: 5-20 parts of OP-10, 10-35 parts of n-octyl alcohol, 30-60 parts of isooctane, 0.1-1.0 part of Tris-HCl buffer solution and 0.1-8.0 parts of distilled water. The reverse micelle emulsion used for the biological enzyme immobilization has the advantages that a surfactant is dispersed into a continuous non-polar solvent, and a nanoscale stable aggregate is spontaneously formed; when the reverse micelle system is taken as a medium for carrying out biological enzyme immobilization, a biological enzyme orientationally exists at an oil-water interface of a reverse micelle, active groups of the biological enzyme are orientationally and regularly toward an organic solvent phase, and groups reacting with nano magnetic particles are regularly toward a water pool, so that loss of the active groups of the biological enzyme is greatly avoided, and the immobilized biological enzyme has efficient catalytic performance; meanwhile, after reaction is finished, the reverse micelle system can be recycled and repeatedly used, so that the reverse micelle emulsion used for the biological enzyme immobilization has a broad development prospect.

Description

technical field [0001] The invention relates to the technical field of biological enzyme immobilization, in particular to a reverse micelle emulsion for biological enzyme immobilization and a method for immobilizing biological enzyme. Background technique [0002] Although biological enzymes have the characteristics of high catalytic activity, strong specificity, and good selectivity, their disadvantages such as high price, difficult recovery, and easy aggregation limit their applications. In order to overcome these shortcomings, more and more researchers have paid more and more attention to the study of immobilizing biological enzymes on carrier nano-magnetic particles. This method has the advantages of simple preparation process, low price, and easy recovery. Among them, the carrier represents nano-Fe 3 o 4 magnetic particles, etc. However, the conventional immobilization method is usually carried out in water, and this method allows the biological enzyme to combine with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/08
CPCC12N11/14C12N9/20C12N11/08C12Y301/01003
Inventor 易世雄高燕代方银孙胜
Owner SOUTHWEST UNIVERSITY
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