A kind of breeding method of the fourth generation of sunflower inbred line Anniversary
An inbred line, sunflower technology, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of weak combination advantages, long breeding cycle, shortage, etc., and achieve the effect of shortening the cultivation cycle
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Embodiment 1
[0021] The concrete steps of the sunflower inbred line anniversary four-generation breeding method that the present invention adopts are as follows:
[0022] (1) The first generation of spring sowing breeding: The selection criteria for spring sowing breeding sites is based on the effective accumulated temperature of ≥5°C within 110 days from the date of sowing ≥1900°C; the seedlings are raised in greenhouse pots in early March, and transplanted to the breeding test field after 30-35 days. Irrigate and fertilize to ensure that the seedlings are neat and strong; in the first ten days of May, the seedlings are bagged for pollination, and 10 days after pollination, the embryos are manually picked up for inoculation and culture, and the first generation is completed.
[0023] (2) The second generation of summer sowing breeding: The selection criteria for summer sowing breeding sites is based on the effective accumulated temperature of ≥5°C within 100 days from the date of embryo in...
Embodiment 2
[0028] A breeding method for the fourth generation of sunflower inbred lines. The first generation is bred in spring in Shihezi City, Xinjiang. On the basis of spring sowing, the second generation is bred by using the technology of young embryo culture in summer; For the breeding of two generations of sunflower inbred lines, the goal of four-generation breeding is achieved. The specific steps of the breeding method are as follows:
[0029] (1) The first generation of transplanting and breeding in spring sowing greenhouse seedlings in Shihezi City, Xinjiang: Seedlings were raised in pots and pots in the greenhouse on March 15, and transplanted to the breeding test field after 30-35 days, watered and fertilized to ensure that the seedlings were neat and strong, and on May 15 The left and right flowers are bagged for pollination, and 10 days after pollination, the embryos are manually picked up for inoculation and culture, that is, the first generation of addition is completed.
...
Embodiment 3
[0034] Using the serial breeding experiments of the fourth generation of sunflower inbred lines in the series of the above-mentioned embodiments of the present invention, 6 generations have been added continuously from March 2013 to September 2014, and stable sunflower restorer lines Y2592R and Y2613R have been bred. The restorer lines are Branch type, plant height 80-90 cm, large amount of pollen, long oval grain, 100-grain weight about 3g, good appearance, good commercial performance, disease resistance, growth period of about 95 days, hybrids XKY1501 and XKY1502 have been configured, thus It can be seen that this method significantly shortens the selection process of sunflower inbred lines, reduces the cost of selection, and has outstanding comprehensive advantages.
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