Biological material for improving release of endothelial progenitor cell exosomes (EPC-EXs) and promoting bone defect repair, and preparation method and application thereof
A technology of endothelial progenitor cells and biomaterials, applied in the field of regeneration and repair of bone injury, can solve problems such as lack of further research, and achieve the effect of promoting release, significant effect, and broad clinical application prospects.
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Embodiment 1
[0016] Example 1: A biomaterial that improves the release of endothelial progenitor cell exosomes and promotes bone defect repair, characterized in that the biomaterial is in situ intermolecular cross-linked by chondroitin oxysulfate (CS) and type I collagen (ColI) The constructed polyelectrolyte multilayer membrane, in which the outermost component is CS; the assembly concentration of CS and ColI are 0.5mg / mL respectively; the oxidation degree of CS is 25%; the assembly conditions are: pH 4.0, ionic strength 0.15 mol / LNaCl, the temperature is room temperature.
[0017] The preparation method of the biological material that increases the release of endothelial progenitor cell exosomes and promotes bone defect repair is characterized in that it is carried out according to the following steps: 1. The culture plate is used as the assembly substrate, and the assembly substrate is first immersed in pH 7.4 at room temperature. In a polyethyleneimine (PEI) solution with a concentration...
Embodiment 2
[0019] The biological material for enhancing the release of endothelial progenitor cell exosomes and promoting bone defect repair, characterized in that the biological material is composed of oxidized chondroitin sulfate (CS) or oxidized hyaluronic acid (HA) and type I collagen (ColI) through in situ The polyelectrolyte multilayer membrane constructed by intermolecular cross-linking, in which the outermost component is CS; the assembly concentration of CS and ColI are 2mg / mL respectively; the oxidation degree of CS is 50%; the assembly conditions are: pH5.0, ionic strength It is 0.15mol / L NaCl and the temperature is room temperature.
[0020] The preparation method of the biological material that increases the release of endothelial progenitor cell exosomes and promotes bone defect repair is characterized in that it is carried out according to the following steps: 1. The culture plate is used as the assembly substrate, and the assembly substrate is first immersed in pH 7.4 at room...
Embodiment 3
[0022] A biomaterial for enhancing the release of endothelial progenitor cell exosomes and promoting bone defect repair, characterized in that the biomaterial is a polyelectrolyte constructed by in situ intermolecular cross-linking of oxidized chondroitin sulfate (CS) and type I collagen (ColI) Multilayer film, where the outermost component is CS; the assembly concentration of CS and ColI are 1.0 mg / mL respectively; the oxidation degree of CS is 40%; the assembly conditions are: pH 4.5, ionic strength 0.15 mol / L NaCl, temperature It is room temperature.
[0023] The preparation method of the biological material that increases the release of endothelial progenitor cell exosomes and promotes bone defect repair is characterized in that it is carried out according to the following steps: 1. The culture plate is used as the assembly substrate, and the assembly substrate is first immersed in pH 7.4 at room temperature. In a polyethyleneimine (PEI) solution with a concentration of 5mg / m...
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