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Compound microbial agent for silage and application thereof in whole-plant corn silage

A technology of compound microbial bacterial agent and whole plant corn, applied in the direction of microorganism-based methods, microorganisms, applications, etc., can solve problems such as high water content, difficult to use, and nutrient loss

Pending Publication Date: 2016-05-11
天津市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, whole plant corn, corn stalks and alfalfa silage are mainly used in my country. Among them, whole plant corn silage technology is relatively mature, but there are still common problems such as high water content and serious nutrient loss; corn stalk silage has reasonable water content but its nutrition The ingredients are relatively poor and difficult to use; alfalfa silage is difficult to silage due to its low water-soluble carbohydrate content and high buffering degree, and its quality is poor, especially the protein loss is serious

Method used

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  • Compound microbial agent for silage and application thereof in whole-plant corn silage
  • Compound microbial agent for silage and application thereof in whole-plant corn silage
  • Compound microbial agent for silage and application thereof in whole-plant corn silage

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Experimental program
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Effect test

Embodiment 1

[0029] Materials and methods:

[0030] Separately prepare the dilutions of Pediococcus lactis, Lactobacillus plantarum and Bacillus coagulans, and mix them at the ratio of mass fraction 1:1:1, 1:1:10 and 1:10:10 respectively so that the total number of colonies is 1.0×10 9 CFU / ml of composite bacterial agents are respectively marked as composite bacterial agent group I, group II and group III.

[0031] The corn at the end of milk ripening was harvested and chopped by a combine harvester, and a 1.5L fermentation tank was prepared, and 1.5kg of each tank was crushed to make silage from the whole plant corn. 100mL / t) + 15g bran (10kg / t) + 13.5g salt (10kg / t), the control group added 150μL distilled water to replace the compound bacterial agent, mixed the additive with the whole corn plant and put it in a fermenter, pressed After 15 days, the sensory (odor, texture and color) evaluation of the silage samples and the determination of various indicators of the silage fermentation q...

Embodiment 2

[0042] Materials and methods:

[0043] Separately prepare dilutions of Pediococcus lactis, Lactobacillus plantarum and Bacillus coagulans, and mix them at a mass fraction of 1:1:1 to obtain a total number of colonies of 5.0×10 9 CFU / ml compound bacterial agent.

[0044] Harvest and chop the corn at the end of milk ripening by a combine harvester, prepare a 1.5L fermenter tank, crush the whole plant corn at a rate of 1.5kg per tank to make silage, and the treatment groups are compound bacterial agent dose I (Pediococcus lactis: Lactobacillus plantarum: Bacillus coagulans=1:1:1) 75μL (50mL / t), compound bacterial dose II150μL (100mL / t) and compound bacterial dose III300μL (200mL / t)+15g bran (10kg / t)+13.5g Salt (9kg / t), the control group added 150μL distilled water to replace the compound bacterial agent, mixed the additive with the whole corn plant and put it in the fermenter, compacted and sealed, and the silage samples were sensory (odor, texture and color) after 15 days. ) e...

Embodiment 3

[0055] Materials and methods:

[0056] Preparation method of compound microbial agent: three strains of Pediococcus lactis (strain preservation number: CGMCC1.4), Lactobacillus plantarum (strain preservation number: ACCC11016), and Bacillus coagulans (strain preservation number: ACCC10229) were activated separately Cultured on MRS liquid medium, 10% inoculum, cultured at 37°C for 48 hours, mixed with MRS medium, mixed with a mass fraction of 1:6:9 to form a composite microbial agent, and the total number of colonies was 9.0×10 9 CFU / ml. All bacterial agents and MRS media are commercially available.

[0057] Method of use: In the process of silage material loading, the compound microbial agent is evenly sprayed on the crushed and timely harvested whole plant corn at an additive dosage of 150mL / t in layers, and bran (10kg / t) is evenly added at the same time and salt (9kg / t), compacted and sealed, and can be opened for use after 15 days, and no compound microbial agent was adde...

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Abstract

The invention discloses a compound microbial agent for silage and an application thereof in whole-plant corn silage. The compound microbial agent is formed by mixing three microbial agents pediococcus acidilactici, lactobacillus plantarum and bacillus coagulans in a mass ratio of 1:(10-1):(10-1), wherein total bacterial count of the compound microbial agent is (1.0-9.9)*10<9>CFU / ml. The 50-200mL / t compound microbial agent is applied to the whole-plant corn silage, the palatability of the silage corn is improved, the loss of dry matters and protein and starch as well as the content of neutral detergent fiber and acid detergent fiber in the whole-plant corn silage are effectively reduced, the proportion of lactic acid in total organic acids is increased while the proportion of acetic acid and propionic acid and butyric acid is reduced, and the silage fermentation quality is improved to certain degree. Through full use of the advantages of microbial fermentation, the compound microbial agent disclosed by the invention has the characteristics of no pollution, high efficiency and low cost, can reduce the nutrient loss of silage material and improve the fermentation quality and finally realizes an effect of effectively storing the silage material.

Description

technical field [0001] The invention discloses a compound microbial bacterial agent for silage and its application in whole-plant corn silage, belonging to the technical field of silage processing in livestock feed. Background technique [0002] Silage is an effective method for long-term preservation of green forage crops. In recent years, it has been used in the field of breeding, especially dairy farming, to store whole corn, corn stalks, sorghum, alfalfa, ryegrass and other forage crops. . Silage has the characteristics of rich nutrition, good palatability, and easy digestion. It can increase the milk production of dairy cows and reduce the occurrence of diseases. At the same time, it can achieve a balanced supply throughout the year to ensure the healthy, rapid and sustainable development of the dairy industry. Important source of high-quality roughage. At present, whole plant corn, corn stalks and alfalfa silage are mainly used in my country. Among them, whole plant ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/12A23K10/30A23K10/37A23K20/22C12R1/07C12R1/25C12R1/01
CPCC12N1/20A23V2400/413A23V2400/169Y02P60/87
Inventor 范寰王丽学霍文娟陈龙宾潘振亮韩静刘景喜冯婧孟繁瑞
Owner 天津市农业科学院
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