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Lactic acid bacteria combination preparation for sugarcane leaf silage and application of lactic acid bacteria combination preparation

A technology of sugarcane caudal leaves and lactic acid bacteria, applied in the direction of application, microbial-based methods, bacteria, etc., can solve problems such as poor aerobic stability, improve fermentation quality, and solve the problem of the ratio of ammoniacal nitrogen to total nitrogen and the content of butyric acid High, improve the effect of dry matter recovery

Active Publication Date: 2018-12-14
广西烨牧生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The appearance score of sugarcane caudal leaves was higher after natural silage, but the aerobic stability was poorer after exposure to air, mainly due to the higher content of ammoniacal nitrogen and butyric acid in the feed of natural silage sugarcane caudal leaves, while ammoniacal nitrogen represented the The decomposition of protein and amino acid in the feed, the smaller the ratio of ammonia nitrogen to the total nitrogen, the better the nutritional value of the feed is preserved

Method used

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  • Lactic acid bacteria combination preparation for sugarcane leaf silage and application of lactic acid bacteria combination preparation
  • Lactic acid bacteria combination preparation for sugarcane leaf silage and application of lactic acid bacteria combination preparation
  • Lactic acid bacteria combination preparation for sugarcane leaf silage and application of lactic acid bacteria combination preparation

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Effect test

Embodiment 1

[0027] A kind of lactic acid bacteria combination preparation that is used for sugarcane tail leaf silage of the present invention, lactic acid bacteria combination has following 4 kinds of forms: (1) Lactobacillus plantarum+Lactobacillus casei+Lactobacillus fermentum, (2) Lactobacillus plantarum+cheese milk Bacillus + Lactobacillus rhamnosus, (3) Lactobacillus plantarum + Lactobacillus fermentum + Lactobacillus rhamnosus, (4) Lactobacillus casei + Lactobacillus fermentum + Lactobacillus rhamnosus.

[0028] For the 4 kinds of lactic acid bacteria combinations used in the lactic acid bacteria combination preparation, the test strains need to be activated and expanded, and the strains in the -80°C glycerol cryopreservation tube are activated, and transferred to the MRS liquid medium at a 3% inoculation amount. Lactobacillus plantarum , Lactobacillus casei, Lactobacillus fermentum and Lactobacillus rhamnosus are cultured at 37°C, and cultured at a static constant temperature for 2...

Embodiment 2

[0032] Using the combination preparation of Lactobacillus plantarum+Lactobacillus casei+Lactobacillus fermentum of the present invention to make silage for fresh sugarcane tail leaves and observe its silage quality

[0033] 1 test material

[0034] Silage raw materials: Fresh sugarcane tail leaves are sourced from the experimental field of the Agricultural College of Guangxi University, and the sugarcane tails are manually cut to a length of 1-4 cm. Variety: New Taiwan Sugar No. 22. The chemical composition of the silage raw sugarcane caudal leaves is shown in Table 1.

[0035] Table 1 Chemical composition of sugarcane tail leaf raw materials (%)

[0036]

[0037] Source of strains: Lactobacillus plantarum, Lactobacillus casei and Lactobacillus fermentum were all screened from sugarcane tail leaves of natural silage, among which Lactobacillus plantarum was preserved in China Type Culture Collection Center on July 2, 2018. The preservation number is CCTCC No: M 2018437; L...

Embodiment 3

[0086] Using the combined preparation of Lactobacillus plantarum+Lactobacillus casei+Lactobacillus rhamnosus of the present invention to make silage for fresh sugarcane tail leaves and observe its silage quality

[0087] 1 test material

[0088] Silage raw materials: Fresh sugarcane caudal leaves were sourced from the experimental field of Agricultural College of Guangxi University, and the sugarcane caudal leaves were manually cut to a length of 1-4 cm. Variety: New Taiwan Sugar No. 22. The chemical composition of the silage raw sugarcane caudal leaves is shown in Table 6.

[0089] Table 6 Sugarcane tail leaf raw material chemical composition (%)

[0090]

[0091] Source of strains: Lactobacillus plantarum, Lactobacillus casei and Lactobacillus rhamnosus were all screened from the tail leaves of sugarcane in natural silage, among which Lactobacillus plantarum was preserved in China Type Culture Collection on July 2, 2018 Center, the preservation number is CCTCC No: M 20...

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Abstract

The invention relates to a lactic acid bacteria combination preparation for sugarcane leaf silage and an application of the lactic acid bacteria combination preparation. The lactic acid bacteria combination preparation is prepared by combination of lactobacillus plantarum, lactobacillus fermentum, lactobacillus rhamnosus and lactobacillus casei in a certain proportion. According to the applicationof the lactic acid bacteria combination preparation to a sugarcane leaf silage feed, the lactic acid bacteria combination preparation is used for silaging sugarcane leaves. The lactic acid bacteria combination preparation provided by the invention is applied to the silaging of the sugarcane leaves, so that the aerobic stability of the sugarcane leaf silage can be effectively improved, the recovery rate of dry matter of the sugarcane leaf silage is increased, the ratio of ammoniacal nitrogen to total nitrogen in the sugarcane leaf silage is reduced, and the content of butyric acid is reduced.

Description

technical field [0001] The invention belongs to the technical field of silage production, in particular to a lactic acid bacteria combination preparation for sugarcane tail leaf silage and its application. Background technique [0002] Guangxi is the main planting area of ​​sugarcane in my country. Winter is the harvest time of sugarcane. There are a large number of tail leaves of sugarcane available. Silage is an effective storage method to preserve the feed nutrients of sugarcane tail leaves. [0003] The dry matter content of sugarcane tail leaves is 28.5%-36.60%, and the crude protein content is 5.13%-5.97%, which is a medium-quality roughage. In Guangxi, the harvesting season of sugarcane lasts from November to April of the next year, which is a period of high humidity in Guangxi. Therefore, it is unrealistic to dry the tail leaves of sugarcane to make hay, and it is easy to cause mildew when stacked. Burning not only It pollutes the air and causes a waste of resources...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K30/18C12R1/25C12R1/245C12R1/225
CPCA23K30/18C12N1/20C12N1/205C12R2001/225C12R2001/245C12R2001/25
Inventor 邹彩霞何仁春穆胜龙韦英明梁明振周俊华林波夏中生
Owner 广西烨牧生物科技有限公司
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