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Ultrasensitive latex-enhanced immunoturbidimetric detection reagent and its detection method and application

A detection reagent and latex-enhanced technology, which is applied in the field of ultra-sensitive latex-enhanced immunoturbidimetric detection reagents and its detection, can solve the problems of poor sensitivity of immunoturbidimetric method, and achieve increased reagent sensitivity, high detection sensitivity, and low detection limit Effect

Active Publication Date: 2018-05-08
SUZHOU DIAGVITA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its disadvantage is that in some detection items, the sensitivity of immunoturbidimetry is poorer in items that require higher sensitivity to protein content in body fluids.

Method used

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  • Ultrasensitive latex-enhanced immunoturbidimetric detection reagent and its detection method and application
  • Ultrasensitive latex-enhanced immunoturbidimetric detection reagent and its detection method and application
  • Ultrasensitive latex-enhanced immunoturbidimetric detection reagent and its detection method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The detection of embodiment 1 procalcitonin (PCT)

[0044]1) Take 180 μl of the reagent reaction solution, and then take an appropriate amount of mouse anti-human monoclonal antibody (specifically, the hytest product number is 14C12 antibody) to obtain the reagent reaction solution labeled with the monoclonal antibody, so that the final concentration of the monoclonal antibody is 50 ng / ml, and then take 20 μl of human serum samples (taken from the Second Affiliated Hospital of Soochow University, and the subject signed a donation statement), repeated 10 times, as a parallel sample, at the same time to take the same amount of procalcitonin standard and Physiological saline is used as a positive control and a negative control respectively, and then the samples taken are respectively added to the reagent reaction solution labeled with the monoclonal antibody, and incubated at 37° for 3 minutes to obtain a reaction mixture;

[0045] 2) Then, take 60 μl of polystyrene latex...

Embodiment 2

[0049] The detection of embodiment 2 procalcitonin

[0050] 1) Take 180 μl of the reagent reaction solution, and then take an appropriate amount of mouse anti-human monoclonal antibody (specifically, the product number of hytest is 14C12 antibody) to obtain the reagent reaction solution labeled with the monoclonal antibody, so that the final concentration of the monoclonal antibody is 40 ng / ml, and then take 20 μl of human serum samples (taken from the Second Affiliated Hospital of Soochow University, and the subject signed a donation statement), repeated 10 times, as a parallel sample, and at the same time to take the same amount of procalcitonin standard and normal saline, respectively, as positive control and negative control, and then the samples taken were added to the reagent reaction solution labeled with the monoclonal antibody, and incubated at 37° for 3 minutes to obtain a reaction mixture;

[0051] 2) Then, take 60 μl of polystyrene latex microspheres, first combin...

Embodiment 3

[0055] The detection of embodiment 3 troponin (cTnI)

[0056] 1) Take 180 μl of the reagent reaction solution, and then take an appropriate amount of mouse anti-human monoclonal antibody (specifically, the hytest product number is M18 antibody) to obtain the reagent reaction solution labeled with the monoclonal antibody, so that the final concentration of the monoclonal antibody is 60 ng / ml, and then take 25 μl of human serum samples (taken from the Second Affiliated Hospital of Soochow University, and the subject signed a donation statement), repeated 10 times, as a parallel sample, while taking the same amount of troponin standard and Physiological saline is used as a positive control and a negative control respectively, and then the samples taken are respectively added to the reagent reaction solution labeled with the monoclonal antibody, and incubated at 37° for 3 minutes to obtain a reaction mixture;

[0057] 2) Then, take 60 μl of polystyrene latex microspheres, first c...

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Abstract

The invention discloses a hypersensitive latex-enhanced immune turbidimetric detection reagent and its detection method and application. The detection reagent includes a reagent reaction solution and polystyrene latex microspheres, and the reagent reaction solution also contains Combined monoclonal antibody; also contain anti-monoclonal antibody in the polystyrene latex microsphere, and described anti-monoclonal antibody is the antibody specifically combined with described monoclonal antibody, and described anti-monoclonal antibody and described Polystyrene latex microspheres cross-linked. The turbidimetric detection reagent antigen in the present invention increases the aggregation site (the monoclonal antibody on the latex particle and its corresponding secondary antibody) in the combination with the antibody, so that the antigen will encounter the labeled particle even when the concentration is very low Rapid agglutination and the formation of larger immune complexes in a shorter time, thus achieving the effect of increasing the sensitivity of the reagent.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a detection reagent, a detection method and application thereof, in particular to an ultrasensitive latex-enhanced immune turbidimetric detection reagent, a detection method and an application thereof. Background technique [0002] Protein is a multimer connected by 20 different L-type amino acids, and its structure can be divided into four levels. The primary structure is a series of amino acid sequences that make up the polypeptide chain of a protein; the secondary structure is a structure formed by hydrogen bonds between different amino acids, and the main types are α-helix and β-sheet; the tertiary structure is spatially separated by secondary structural units The three-dimensional structure of the protein formed; the quaternary structure is the interaction between different subunits to form protein complex molecules with specific functions. [0003] Immunolabelin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/324
Inventor 王滨贾引军辛杰吴一凡
Owner SUZHOU DIAGVITA BIOTECH
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