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Metarhizium anisopliae and its application to control the palm pest

A technology of coconut weaver moth and Metarhizium anisopliae is applied in the field of microorganisms to achieve good insecticidal effect, reduce loss and good insecticidal effect.

Inactive Publication Date: 2018-11-13
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieval, no related reports have been found on the use of Metarhizium anisopliae to control the cocoon moth

Method used

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  • Metarhizium anisopliae and its application to control the palm pest
  • Metarhizium anisopliae and its application to control the palm pest
  • Metarhizium anisopliae and its application to control the palm pest

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1 Screening and Identification of Bacterial Strains

[0017] 1. Screening of bacterial strains

[0018] Take the collected samples of the cocoon moth worm, first disinfect them with 70% alcohol for one minute, wash them with sterile water, and then use 0.1% mercuric chloride to disinfect the surface of the susceptible samples for 30 seconds, wash them three times with sterile water, and pick them up. Take a little zombie tissue and inoculate it in selective SDAY medium (4% glucose, 1% peptone, 1% yeast powder, 2% agar, 100 μg / ml streptomycin, 100 μg / ml penicillin, carbendazim 0.8 μg / ml) , placed upside down at a constant temperature of 28±1°C for 3-4 days, select single spores to inoculate in PDA medium (200g peeled potatoes, cut into small pieces, add water to 1L, boil for 20 minutes, then filter with double gauze, add 20g glucose and 20g agar to the filtrate Reheat to make it melt, add water to make up the volume to 1L) Purify, 3 points per dish, arranged in ...

Embodiment 2

[0026] Example 2 Determination of Indoor Insecticidal Activity of Bacterial Strains to Coconut Weaver Moth

[0027] Take the isolated and purified Metarhizum anisopliae LSY-1 strain, put the conidia powder into a 50ml sterile centrifuge tube, add 10mL 0.1% Tween 80 sterile water, and use a vortex shaker to Shake for 15 minutes to mix well, then measure the number of spores per milliliter of suspension with a hemocytometer, and adjust the strain so that the concentration of spores is 1.0×10 8 per mL, and stored in a refrigerator at 10°C for future use. The spores of Metarhizumanisopliae (Metarhizumanisopliae) LSY-1 bacterial strain were made into 5 concentrations of water preparations in a gradient dilution method, and the concentrations were 1.0×10 8 , 5×10 7 , 2.5×10 7 , 1.25×10 7 , 6.25×10 6 pieces / ml. Cut the fresh old leaves into leaf fragments of about 8cm, then put the leaf fragments into 10ml spore suspension to fully smear and infiltrate, put them into sterilized...

Embodiment 3

[0030] Example 3 Outdoor control effect of bacterial strains

[0031] Bring the water agent of Metarhizum anisopliae (Metarhizum anisopliae) LSY-1 bacterial strain to field, dilute to contain conidia 1×10 8 pcs / mL, and stirred evenly, ultra-low volume spray. Select 3 coconut branches with similar damage degree, surround the branch ends of the damaged coconuts with insect nets and tighten the insect nets, and control 30 coconut weaver moth larvae for each treatment by picking out some test insects. Spray control started on November 5, 2015. At that time, the weather was cloudy and cloudy, the temperature was 27°C, the wind force was level 1, and the air humidity was 82%. Average coverage density 40.5 drops / cm 3 . Check every 2 days, the results are shown in Table 2. It can be seen from the results that after 4 days of processing, the survival rate of larvae in the trunk began to decline significantly, and the death rate was 96.67% after 14 days, showing that Metarhizum anis...

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Abstract

The invention relates to metarhizum anisopliae for preventing and controlling palm pest opisina arenosella walker and application of the metarhizum anisopliae. The strain is named as metarhizum anisopliae LSY-1 and is preserved in China Center for Type Culture Collection on November 9, 2015 with preservation number of CCTCC M 2015672. The metarhizum anisopliae LSY-1 provided by the invention is applicable to the prevention and control of the opisina arenosella walker; a test of direct smearing administration shows that the metarhizum anisopliae LSY-1 has a good insecticidal action on the palm pest opisina arenosella walker and the metarhizum anisopliae can be used for effectively controlling the population quantity of the opisina arenosella walker; and the metarhizum anisopliae, as a bio-control strain which has a potential significance to prevent and control the opisina arenosella walker, can offer help for the development of the palm industry in China.

Description

technical field [0001] The invention belongs to the field of microbes, and relates to a metarhizium anisopliae and its application, in particular to a metarhizium anisopliae for preventing and controlling palm pests, the palm moth, and its application. Background technique [0002] Coconut weaving moth Opisina arenosella Walker (also known as coconut wood moth, black-headed caterpillar, coconut column moth, leaf-eating caterpillar), belongs to Lepidoptera (Lepidoptera) weaving moth family (Qecophoridae), English name coconut blackheaded caterpillar, mainly distributed in India , Thailand, Indonesia, Malaysia, Sri Lanka, Bangladesh, Myanmar, Pakistan and other places, it is a kind of alien invasive pest that seriously harms palm crops such as coconut, betel nut, palmetto, Middle East date palm and king palm. It was listed as a national pest in March 2014. Forestry dangerous pests. Coconut weaver moth larvae feed on leaves, and in severe cases, the entire canopy is invaded an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 孙晓东阎伟覃伟权吕朝军李朝绪
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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