Microfluidic device and method for optimal control on microbial fertilizer bacterium culture conditions

Abstract

The invention discloses a microfluidic device and method for optimal control on microbial fertilizer bacterium culture conditions. The device comprises a case body and a PC (personal computer) positioned outside the case body, wherein a gas pump, a 10-channel constant-pressure sampler, an objective table, a microcontroller and a three-stage microfluidic chip are fixedly arranged in the case body. A complete optimal control method of nutrient solution main component screening, main component concentration proportion optimization and sample introduction parameter and temperature optimization of the optimal proportion nutrient solution is adopted to implement the microfluid intelligent control, temperature control and intelligent monitoring double feedback and also implement microfluid intelligent control, external shape image automatic treatment and interior physiologic metabolite activity electrochemical detection double feedback, thereby directly controlling the multichannel sample introduction mode and sample introduction parameters to perform the microfluidic microbial fertilizer bacterium culture. The method can be used for culture condition optimal control on any microbial fertilizer bacterium under the condition of unknown nutrient solution components.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a microfluidic chip device and method for optimal control of microbial fertilizer culture conditions, and also relates to computer communication, automated electronic technology, biochemistry and other multidisciplinary fields. Background technique [0002] With the rapid development of modern agriculture, the use of chemical fertilizers is increasing year by year. The traditional organic fertilizer resources are in short supply, the preparation process consumes energy and materials, the fertilizer utilization rate is low, the soil fertility decreases, the yield and quality of crops are greatly reduced, the environmental pollution is serious, and the ecological balance is destroyed. Compared with traditional organic fertilizers, microbial fertilizers have sufficient resources and high utilization rates, which can improve and maintain the transformation of nutrien...

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Problems solved by technology

[0004] The purpose of the present invention is to propose a microfluidic microfluidic device for optimal control of microbial fertilizer culture conditions in view of the shortcomings of existing microfluidic cell culture devices and methods such as single function, low integration, and low intelligence. The control device and method can optimize the main components of the nutrient solution, optimize the concentration ratio of the main components, temperature, etc., and realize intelligent control and intelligent monitoring

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