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A palladium nanoparticle with peroxidase activity and preparation method thereof

A technology of palladium nanoparticles and peroxidase, applied in chemical instruments and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve the problem of low peroxidase activity, difficult conditions to control, and cumbersome preparation and other problems, to achieve the effect of simple method, easy control of conditions and high catalytic activity

Active Publication Date: 2017-11-07
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its preparation is cumbersome, the cycle is long, the conditions are not easy to control, and the peroxidase activity is low

Method used

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  • A palladium nanoparticle with peroxidase activity and preparation method thereof
  • A palladium nanoparticle with peroxidase activity and preparation method thereof
  • A palladium nanoparticle with peroxidase activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A preparation method of palladium nanoparticles with peroxidase activity, comprising the steps of:

[0036] (1) Deoxynucleotide C 10 The annealing of:

[0037] Dissolve 25 μL of 20 μM deoxynucleotide C 10 Add aqueous solution, 100 μL 10 mM sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution with a pH of 5.0 to a 1.5 mL centrifuge tube, add triple distilled water to make the total volume 400 μL, and mix well; room temperature;

[0038] (2) Preparation of palladium nanoparticles:

[0039] Add 12 μL of 300 μM sodium chloropalladate aqueous solution to the annealed deoxynucleotide aqueous solution obtained in step (1), mix well at 25°C, let stand for 2 hours, add 15 μL 1.5mM dimethylamine borane aqueous solution, mix well, Standing at 25° C. for 12 h, a palladium nanoparticle liquid with peroxidase activity was obtained. The average particle size obtained by scanning with a transmission electron microscope is 4.8nm. (See figure 1 )

[0040] Detec...

Embodiment 2

[0047] A preparation method of palladium nanoparticles with peroxidase activity, comprising the steps of:

[0048] (1) Deoxynucleotide G 200 The annealing of:

[0049] Dissolve 2.5 μL of 2 μM deoxynucleotide G 200 Aqueous solution, 50 μL of 5 mM potassium dihydrogen phosphate-dipotassium hydrogen phosphate buffer solution with a pH of 3.0 was added to a 1.5 mL centrifuge tube, and triple distilled water was added to make the total volume 400 μL. room temperature;

[0050] (2) Preparation of palladium nanoparticles:

[0051] Add 8 μL of 200 μM sodium chloropalladate aqueous solution to the annealed deoxynucleotide aqueous solution obtained in step (1), mix well at 20°C, let stand for 4 hours, add 6 μL 0.6mM sodium borohydride aqueous solution, mix well, and Stand still at ℃ for 15 hours to obtain palladium nanoparticle liquid with peroxidase activity.

[0052] The average particle size obtained by scanning with a transmission electron microscope is 1.0 nm. The determinati...

Embodiment 3

[0054] A preparation method of palladium nanoparticles with peroxidase activity, comprising the steps of:

[0055] (1) Deoxynucleotide C 200 The annealing of:

[0056] Dissolve 250 μL of 200 μM deoxynucleotide C 200Add aqueous solution, 200 μL 20 mM sodium dihydrogen phosphate-phosphoric acid solution with a pH of 9.0 to a 1.5 mL centrifuge tube and add triple distilled water to make the total volume 400 μL, mix well; heat up to 100 °C, maintain for 5 min, and cool down to room temperature;

[0057] (2) Preparation of palladium nanoparticles:

[0058] Add 16 μL of 400 μM sodium chloropalladate aqueous solution to the annealed deoxynucleotide aqueous solution obtained in step (1), mix well at 30° C., let stand for 0.5 h, add 24 μL 2.4 mM dimethylamine borane aqueous solution, mix well, Standing at 30° C. for 10 h, a palladium nanoparticle liquid with peroxidase activity was obtained.

[0059] The average particle size obtained by scanning with a transmission electron micros...

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Abstract

The invention discloses a palladium nanoparticle with peroxidase activity and a preparation method thereof. The preparation method comprises the following steps: (1) annealing of deoxynucleotides: adding deoxynucleotide aqueous solution and phosphate buffer solution to 1.5 mL and add it into the centrifuge tube, mix well; raise the temperature to 90-100°C, maintain it for 5-10min, and cool down to room temperature; (2) Preparation of palladium nanoparticles: Add sodium chloropalladate aqueous solution to the annealed mixture obtained in step (1). In the deoxynucleotide aqueous solution, mix well, stand still, add reducing agent aqueous solution, mix well, stand still, obtain the palladium nanoparticle liquid with peroxidase activity. The method of the invention is simple, short in cycle, easy to control and mild in conditions, and is convenient for large-scale industrial production and preparation; the average particle diameter of the palladium nanoparticles with peroxidase activity is 1.0-4.8nm, and the catalytic activity is high.

Description

technical field [0001] The invention belongs to the technical field of nanoparticle preparation, and in particular relates to a preparation method of palladium nanoparticle with peroxidase activity. Background technique [0002] Palladium nanomaterials have been recognized as a highly ideal catalyst, and its catalytic activity has a great relationship with surface geometric effects, electronic properties and quantum size effects. So far, palladium nanoparticles have been prepared with carbon, metal oxides, dendrimers, block copolymer micelles, mesoporous molecular sieves, etc. as support materials. Its preparation is cumbersome, the cycle is long, the conditions are not easy to control, and the activity of peroxidase is low. Therefore, it is of great practical significance to develop a convenient and effective preparation method of palladium nanoparticles with peroxidase activity. Contents of the invention [0003] The purpose of the present invention is to provide a met...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B22F1/00B01J23/44B01J35/00B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00B01J23/44B22F9/24B22F1/054B01J35/393
Inventor 付雁李韡张金利王雨智星
Owner TIANJIN UNIV
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