A method for the reproduction and preservation of endangered sheep deer in vitro tissue culture

Abstract

The invention discloses a method for in vitro tissue culture, reproduction and preservation of endangered sheep roe deer, establishes the best rapid propagation system and preservation system in the tissue culture process of sheep roe deer, including the most suitable selection method for explants, and is highly efficient. The multi-sterilization method, the most suitable medium composition for the in vitro growth and preservation of the sheep hemp , High-quality reproduction. The invention can make the differentiation rate of the test-tube seedlings of the goat deer reach up to 100%, the multiplication factor of the adventitious buds reaches 8-10.5 per month, and the rooting rate and domestication survival rate after 40 days are both over 85%. This can effectively meet people's increasing demand for sheep roe, thereby reducing people's damage to wild sheep roe, which is conducive to the protection of endangered wild sheep roe, and has great scientific research value, economic value and ecological value.

Description

technical field [0001] The invention belongs to the technical field of plant biotechnology and cell engineering, and relates to a method for in vitro tissue culture, reproduction and preservation of endangered sheep deer. Background technique [0002] Rhododendronmolle (Rhododendronmolle G.Don), also known as Naoyanghua and Balima, is the only native species in the subgenus Rhododendronmolle in China. It blooms in spring and has bright yellow flowers. It can be used for viewing. It is a rhododendron molle color variety. An important resource for improvement. In addition, the organs of the whole plant of the goat roe contain various active ingredients, and the flowers, roots, and fruits are all available for medicinal purposes. Its extracts can treat rheumatoid, febrile malaria, chronic glomerulonephritis, and hypertension. It is used as an anesthetic and analgesic in industry, and used as an insecticide in agriculture. It has a very good killing effect on schistosomiasis an...

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for in vitro tissue culture, reproduction and preservation of endangered sheep roe deer, aiming at the sharp reduction in the area and population of sheep roe deer distributed in the wild, and the small seeds are difficult to germinate under natural conditions, and artificially Propagation by cuttings or divisions is also difficult to survive, there are explant sterilization difficulties in the tissue culture process, the acquisition rate of sterile explants is low, and the explants are difficult to differentiate and multiply. The present invention provides an endangered The method of in vitro large-scale reproduction and preservation of sheep roe deer provides technical support for realizing large-scale, rapid and high-quality reproduction of sheep roe deer in a short period of time, and provides tissue culture seedlings to meet people's growing demand for sheep roe deer, Provide technical support and reference for the protection of wild endangered sheep roe

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