The function and application of osjmj714 affecting rice grain size and salt stress tolerance

A technology of JMJ714, rice grain, applied in the direction of application, introduction of foreign genetic material and enzymes using vectors, etc., can solve the problem that the impact of research on crop yield is poorly understood.

Inactive Publication Date: 2018-12-07
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the synthesis, transport and signal transduction of auxin have been well studied in the model plant Arabidopsis thaliana, its research in crops and its impact on crop yield are still poorly understood

Method used

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  • The function and application of osjmj714 affecting rice grain size and salt stress tolerance
  • The function and application of osjmj714 affecting rice grain size and salt stress tolerance
  • The function and application of osjmj714 affecting rice grain size and salt stress tolerance

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Creation and identification of transgenic rice materials

[0019] 1. Construction of OsJMJ714 gene recombinant plant expression vector

[0020] Using the coding region sequence of OsJMJ714 (gene number: Os09g0483600) as a template, OsJMJ714-F 5'-AGTGGATCCATGGAGCGCGCAGTGCGGGA-3' and OsJMJ714-R 5'-GGCGGTACCTCAATCATTCGTCTCCTCGA-3' were designed by bioinformatics analysis. The 1080bp full-length coding region sequence fragment of OsJMJ714 was amplified by PCR from the cDNA library, and the PCR fragment and the plant expression vector pCAMBIA1205 were digested with BamHI and KpnI, then ligated and transformed into E. coli DH5α, and positive clones were picked Identification and sequencing analysis were carried out to obtain the recombinant plant expression vector pCAMBIA1205-JMJ714 inserted into the OsJMJ714 gene and in the correct reading frame, which was introduced into Agrobacterium AGLO.

[0021] Creation of rice material to knock out OsEAR factor in rice usi...

Embodiment 2

[0035] Example 2: JMJ714 affects rice grain size

[0036] Rice grain size is closely related to its yield and quality traits. We found that rice grains overexpressed JMJ714 became smaller, as figure 2 As shown, compared with wild-type seeds, JMJ714-overexpressed transgenic rice seeds became smaller, including reduced seed length and width compared with wild-type seeds; 50 mature seeds were further selected for statistical analysis of grain size, wild The average length of the grains of the two transgenic lines was 0.7 cm, 0.61 cm and 0.60 cm; the width of each grain of the wild type was 0.332 cm, and that of the two transgenic lines were 0.294 cm and 0.289 cm respectively. These results indicated that the gene It is involved in the regulation of rice grain size, and its overexpression leads to smaller rice seeds and affects rice yield; while the seeds of transgenic rice with silenced expression of this gene become larger, indicating that reducing the expression of this gene ...

Embodiment 3

[0037] Example 3: Overexpression of OsJMJ714 reduces the salt tolerance of rice

[0038] We treated 3-week-old WT and JMJ714-overexpressed transgenic rice materials with 1% NaCl for 8 days, then removed the saline, and resumed culture in water for 7 days. The results were as follows: image 3 As shown, almost all the transgenic rice plants with JMJ714 overexpression died, while some (about 35%) of WT survived, but most of the transgenic rice plants (about 60%) with the gene silenced survived, indicating that JMJ714 overexpressed Expression results in reduced tolerance of rice to salt stress, and reduced expression of this gene can increase the salt tolerance of transgenic rice.

[0039] Institute of Biotechnology, Chinese Academy of Agricultural Sciences

[0040] The function of OsJMJ714 affecting rice grain size and salt stress tolerance and its application

[0041] 2

[0042] 1

[0043] 1080

[0044] DNA

[0045] 1

[0046] 1 ATGGAGCGCG CAGTGCGGGA GCTGTGGGCG GAGTC...

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Abstract

The invention discloses an application of a rice JMJC family member OsJMJ714 to the influence on the rice grain size and the tolerance to the salt stress. OsJMJ714 transgenic rice is created through a transgenic technology, and the obtained transgenic plant is changed in grain size and salt stress tolerance, so that the OsJMJ714 gene has a very high application value in an aspect of improving agronomic characters of rice, such as the grain size and the stress tolerance.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a gene for regulating rice grain size and salt stress tolerance and its application in crop transgenic improvement. Background technique [0002] Seed size is one of the important factors determining rice yield. It has long been an important goal of many crop breeding and improvement, and its regulatory mechanism has attracted much attention. As one of the most important plant hormones, auxin participates in many important processes of plant growth and development. Although the synthesis, transport and signal transduction of auxin have been well studied in the model plant Arabidopsis thaliana, its research in crops and its effect on crop yield are still poorly understood. Mitogen-activated protein kinases (MAPKs) are ubiquitous protein kinases in organisms, and they play an important role in plant growth and development and stress response. The Institute of Genetics and Developmental...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/82A01H5/00A01H6/46
CPCC12N9/0071C12N15/8261C12N15/8273C12Y114/11
Inventor 张海文张执金权瑞党王娟黄荣峰
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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