High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum

A technology of organic waste and decomposed bacteria agent, applied in the preparation and application of organic fertilizers, organic fertilizers, etc., can solve the problems of low microbial activity, unstable temperature, long fermentation cycle, etc., achieve good microbial metabolic activity and convenient preparation , the effect of accelerating the composting process

Active Publication Date: 2016-04-27
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the application defects of the existing decomposing agent in the treatment of organic waste, aiming at the problems of slow temperature rise, unstable temperature, low microbial activity and long fermentation cycle, etc., to provide a kind of decomposing agent that can withstand high temperature environment, and Decomposing bacteria agent for organic waste with good biological enzyme activity

Method used

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  • High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum
  • High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum
  • High-temperature-resistant decomposing microbial inoculum of organic wastes and application of high-temperature-resistant decomposing microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Screening and Identification of Thermostable Bacillus H1-7 Provided in the Examples of the Present Invention

[0022] Sampling was taken from natural compost and activated sludge, and microorganisms were isolated by serial dilution coating method. Place the diluted coated plate in a 55°C incubator for constant temperature incubation for 24h. Select the faster-growing strains to isolate and purify. Spot the isolated and purified strains on starch plates, protein plates and cellulose Congo red plates with an inoculation needle. Observe the transparent circle after culturing for 24 hours. Carry out primary screening by measuring transparent circle and bacterial colony diameter ratio (seeing table 1), carry out liquid enzyme production fermentation re-screening after primary screening, obtain 8 strains degrading starch, protein, cellulose effect better bacterium (seeing table 2) from compost ), among them, the H1-7 strain has significantly better enzyme produc...

Embodiment 2

[0032] Example 2 Application of the high temperature-resistant bacteria H1-7 provided by the example of the present invention in the mixed composting of kitchen waste and straw

[0033] 1. Food waste straw mixed composting process

[0034] Mix food waste and straw (corn stalks, rice stalks) according to the wet substrate ratio of 5:1, or adjust the C / N ratio according to the physical and chemical properties, so that the C / N ratio of the mixed material is 25-35.

[0035] In the method for preparing stacking materials, the particle size of the kitchen waste may be 2-3 cm; the particle size of the filler (rice stalk, corn stalk, etc.) may be 1-3 cm.

[0036] Mix the fermentation liquid of H1-7 and the commercially available decomposing agent at a mass ratio of 1:10, and insert it into the food waste pile at the inoculation amount of 0.2%. The length, width and height of the pile are 1.5m×1.5m×1.0 m, the moisture content of the heap is maintained at 60-65%, and it can be decompos...

Embodiment 3

[0056] Example 3 Application of the high-temperature-resistant bacteria H1-7 provided by the examples of the present invention in the composting of fruit and vegetable waste

[0057] Apply the high-temperature-resistant bacterial agent in the present invention to fruit and vegetable waste composting, select oyster mushroom residue as an auxiliary material, mix the fruit and vegetable waste with the auxiliary material according to the range of C / N of 25-30, and maintain the moisture content at 55%- Between 65%, inoculate the commercially available decomposing agent with the stacking material, after fully mixing, compound the H1-7 fermentation liquid described in the present invention and the commercially available decomposing agent according to the mass ratio of 1:20, and inoculate according to the inoculation amount of 0.2% Put it in the pile, mix it well, turn it every 3-4 days, and it will be completely decomposed in 12-15 days. The degradation rate of organic matter increas...

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Abstract

The invention discloses a high-temperature-resistant decomposing microbial inoculum for decomposing organic wastes. The high-temperature-resistant decomposing microbial inoculum is bacillus subtilis H1-7, and is collected in CCTCC (China Center for Type Culture Collection), and the collection number is CCTCC NO: M2015174. According to an application method of the H1-7 microbial inoculum to the organic waste decomposing, fermentation liquid of the H1-7 microbial inoculum and a market organic waste decomposing agent are mixed according to a mass ratio of 1: (10-20); the mixture is inoculated into an organic waste pile for fermentation at the inoculation quantity of 0.2 percent to 0.5 percent; and the organic waste decomposing speed can be accelerated. The high-temperature-resistant decomposing microbial inoculum provided by the invention has the advantages that the composition is simple; the preparation is convenient; the popularization is facilitated; a good degradation effect can be maintained in high-temperature environment; high environment adaptability is realized; and the composting progress is accelerated.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and waste resource utilization, and in particular relates to a high-temperature-resistant decomposing microbial agent capable of rapidly decomposing organic waste, a preparation method and application thereof. Background technique [0002] Organic waste includes crop straw, livestock and poultry manure, municipal solid waste, sludge, etc. my country is a large agricultural country, with an annual output of more than 4 billion tons of agricultural waste and livestock and poultry manure, and an annual output of municipal solid waste of more than 250 million tons. Such a huge renewable resource has not been fully utilized. [0003] At present, high-temperature aerobic composting has gradually become an important way to treat organic waste, which can achieve harmlessness, reduction and resource utilization. Scholars at home and abroad have carried out a lot of work on composting maturation inocu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/125
CPCY02W30/40
Inventor 陈守文冀志霞张健威周尚峰徐迪红马昕
Owner HUBEI UNIV
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