Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Process method for separating and purifying bitespiramycin

A technology of bitspiramycin and process method, which is applied in the field of separation and purification of antibiotics, can solve the problems of many types of components and impurities, instability of fermentation broth, etc., achieve orderly crystal forms, clear control indicators, and eliminate local excessive The hidden effect of alkali

Active Publication Date: 2016-04-27
EAST CHINA UNIV OF SCI & TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the solvent extraction method has no specificity for selecting a single component of bitspiramycin. In addition, there are many types of components and impurities, and the instability of the fermentation broth. It is very difficult to produce qualified bitspiramycin finished products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Process method for separating and purifying bitespiramycin
  • Process method for separating and purifying bitespiramycin
  • Process method for separating and purifying bitespiramycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Three-stage countercurrent washing: the butyl acetate extract containing the titrepiromycin titer of 7234u / ml was washed with 0.8% NaH 2 PO 4 The solution was washed countercurrently by a three-stage centrifuge. The washing temperature was 20° C., and the washing phase was water phase: ester phase = 1:1.5, so that the after-washing solution of bitspiramycin with an ester phase titer of 5593u / ml was obtained.

[0050] (2) Buffer back extraction: the separated butyl acetate washing liquid is 0.7% NaH with pH=2.2, mass fraction 2 PO 4 Solution stripping, the stripping phase is the water phase: ester phase = 1:4.5, the water and ester are mixed and stirred, and the water phase is mixed with H 3 PO 4 Adjust the pH of the water phase to 3.60, let stand to separate the liquids, and obtain the back-extraction solution of Bitespiramycin with a titer of 21708u / ml in the water phase.

[0051] (3) Butyl acetate extraction: adjust the pH of the aqueous phase phase extract o...

Embodiment 2

[0056] (1) Three-stage countercurrent washing: the butyl acetate extract containing the titrepiromycin titer of 9078u / ml was washed with 1% NaH 2 PO 4 The solution was washed countercurrently by a three-stage centrifuge. The washing temperature was 20° C., and the washing phase was water phase: ester phase = 1:1.5, so that the after-washing solution of bitspiramycin with the titer of the ester phase was 5249u / ml was obtained.

[0057] (2) Buffer stripping: the separated butyl acetate washing liquid is washed with 0.7% NaH of pH=2.2 2 PO 4 Solution stripping, the stripping phase is the water phase: ester phase = 1:4, the water and ester are mixed and stirred, and the water phase is mixed with H 3 PO 4 Adjust the pH of the water phase to 3.85, let stand to separate the liquids, and obtain the back-extraction solution of Bitespiramycin with a titer of 20468u / ml in the water phase.

[0058] (3) Butyl acetate extraction: adjust the pH of the aqueous phase extract of bispirulin...

Embodiment 3

[0063] (1) Three-stage countercurrent washing: the butyl acetate extract containing the titrepiromycin titer of 8578u / ml was washed with 0.9% NaH 2 PO 4 The solution was washed countercurrently by a three-stage centrifuge. The washing temperature was 20° C., and the washing phase was water phase: ester phase = 1:1.5, so that the after-washing solution of bitspiramycin with an ester phase titer of 6091u / ml was obtained.

[0064] (2) Buffer stripping: the separated butyl acetate washing liquid is washed with 0.7% NaH of pH=2.2 2 PO 4 Solution stripping, the stripping phase is the water phase: ester phase = 1:3.5, the water and ester are mixed and stirred, and the water phase is mixed with H 3 PO 4 Adjust the pH of the water phase to 3.75, let stand to separate the liquids, and obtain the back-extraction solution of Bitespiramycin with a titer of 20972u / ml in the water phase.

[0065] (3) Butyl acetate extraction: adjust the pH of the aqueous phase phase extract of Bitespira...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a process method for separating and purifying bitespiramycin. The method comprises the following steps: (1) an ester extract liquid containing bitespiramycin is subjected to three-stage countercurrent washing, such that a washed ester solution is obtained; (2) the washed ester solution is subjected to first stripping with a phosphate solution, such that primary water extract containing bitespiramycin is obtained; (2) the primary water extract is extracted with ester, such that an ester extract liquid with concentrated bitespiramycin is obtained; (4) the ester extract liquid is subjected to secondary stripping with a phosphate solution, such that secondary water extract containing bitespiramycin is obtained; and (5) ethanol is added; and lye is dropped in for crystallizing, such that a bitespiramycin finished product is obtained. Compared with prior arts, the method provided by the invention adopts three-stage countercurrent extraction washing and a multiple-time solvent extraction method with variable phase ratios, such that impurities with relatively high polarity can be removed, and the effective component can be enriched. Ethanol is added for regulating crystalline system polarity. Low-concentration lye is used for regulating crystalline liquid pH level. With the method, impurities among the components can be effectively removed, and effective component can be enriched.

Description

technical field [0001] The invention relates to a process method for separating and purifying bitspiramycin, which belongs to the technical field of antibiotic separation and purification. Background technique [0002] Macrolides antibiotics are one of the most widely used antibacterial drugs. The parent molecular structure is a 14-16-membered ring macrolide, and the hydroxyl group on the lactone ring is bonded to 1-3 molecules of sugar or amino sugar linked. More than 100 such antibiotics have been discovered so far, and the commonly used ones are: 14-membered ring erythromycin (Erythromycin) and its derivatives Roxithromycin (Roxithromycin), clarithromycin (Clarithromycin), 15-membered ring red Mycin derivatives Azithromycin, the 16-membered ring mainly includes Josamycin, Kitasamycin, Midecamycin, Platenomycin, Tylos Tylosin, Spiramycin and its derivatives. [0003] Bitespiramycin, also known as Kelimycin and Shengjimycin, is a multi-component mixture composed of acyla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H17/08C07H1/06
CPCC07H1/06C07H17/08
Inventor 陈葵朱家文刘慧牛梦旗应喜平杨生武
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com