Porcine oocyte in vitro maturation medium and methods of preparation and culture
A technology for in vitro maturation culture and oocyte, which is applied in the field of culturing pig oocytes, in vitro maturation medium of pig oocytes and their preparation, which can solve the problems of low maturation rate and achieve the effects of improving maturation rate and simple operation.
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[0040] The formulation of the balanced oocyte in vitro culture solution (invitromature, IVM solution) used in the present embodiment is as follows:
[0041]
[0042] Balance of culture solution: Add 1mL of the above-mentioned IVM solution to a 60cm glass petri dish to make it cover the bottom, then cover the surface of the liquid with 500μL of mineral oil, and place in 5% CO 2 The incubator with saturated humidity was equilibrated for at least 6 hours.
[0043] Oocyte collection: Use a 10mL syringe to provide negative pressure to extract eggs, select 4-7mm follicles, and insert the needle in parallel. During the collection process, it is necessary to avoid inserting into the medulla, and extract too many tissue fragments, which will make it difficult to collect eggs and delay the time of egg collection. When picking oocytes, use a simple egg picking device. One end of the silicone tube is tightly covered with an egg picking needle, and the other end is tightly set with a 1...
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