Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Liver cancer gene detection method, detection kit and application thereof

A technology of gene detection and kit, which is applied in the field of liver cancer-related gene detection kit and liver cancer-related gene detection, can solve the problems of patient death, poor pertinence, poor curative effect, etc., and achieve the effect of accurate clinical diagnosis

Inactive Publication Date: 2016-04-20
CHINESE NAT HUMAN GENOME CENT AT SHANGHAI
View PDF0 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current chemotherapy drugs for liver cancer are basically the same as those for other tumors, which are poorly targeted and curative. The cause of death is mainly related to its highly metastatic characteristics. It often invades intrahepatic blood vessels and then metastasizes throughout the body, resulting in the death of the patient.
However, the molecular mechanism leading to the metastasis of liver cancer is unclear, especially the inherent mutation genes that determine the metastasis of tumor cells have not been identified

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liver cancer gene detection method, detection kit and application thereof
  • Liver cancer gene detection method, detection kit and application thereof
  • Liver cancer gene detection method, detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Targeted capture sequencing of two liver cancer patients

[0022] The sequencing method includes the following steps:

[0023] 1. Four tissue samples T1, P1, T2, P2 (T represents cancer tissue, P represents normal para-cancer tissue) were collected from two cases of patients undergoing liver cancer surgery. They were stored in a -80°C refrigerator, and the samples were from Nanjing. Department of Hepatobiliary Surgery, Jiangsu Provincial People's Hospital), for tissue DNA extraction. In this experiment, DNeasyBlood&TissueKit (Qiagen, 69506) kit was used, and the DNA extraction and purification of samples were performed according to the standard operating procedure provided by the manufacturer. DNA via ND-1000 spectrophotometer and 0.8% agarose gel electrophoresis quality inspection were used for later use. The DNA quality inspection results are shown in Table 1.

[0024] Table 1 The quality evaluation results after DNA extraction in two pairs of samples (T...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a liver cancer gene detection kit, which comprises 97 target probes matching with the exon regions of 94 liver cancer associated genes and 3 promoter regions of TERT gene. The invention also discloses application of the kit in diagnosis of liver cancer, and a method for detection of liver cancer associated genes with the kit. The liver cancer gene detection kit provided by the invention can be used for clinical diagnosis and classification of liver cancer, makes liver cancer diagnosis rapider and more accurate, and provides a new way for personalized treatment of liver cancer.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a detection kit for liver cancer-related genes, the application of the kit in the diagnosis of liver cancer, and a method for using the kit to detect liver cancer-related genes. Background technique [0002] Liver cancer is a serious threat to the life and health of our people. The diagnosis of liver cancer, especially early diagnosis is the key to clinical diagnosis, treatment and prognosis. The treatment of liver cancer has made great progress in the past 20 years. Surgical resection and liver transplantation are "radical" treatments and are still the first choice for the treatment of liver cancer. With the improvement of the early diagnosis level of liver cancer and the change of the biological concept of tumor treatment, the status of non-surgical local tumor treatment in the treatment of liver cancer is increasing day by day. Transcatheter arterial chemoembolization (TACE) ha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68
Inventor 韩泽广胡涛涛
Owner CHINESE NAT HUMAN GENOME CENT AT SHANGHAI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com