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Rolling circle amplification-terahertz metamaterial biosensor for parallel detection of multiple pathogenic bacteria and its detection method

A biosensor, rolling circle amplification technology, applied in biochemical equipment and methods, bioreactors/fermenters for specific purposes, determination/inspection of microorganisms, etc. Responsive and responsive effect

Active Publication Date: 2017-09-29
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Abstract
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  • Application Information

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Problems solved by technology

However, at present, RCA is mainly detected by agarose gel electrophoresis, which has a long cycle and poor stability of results.

Method used

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  • Rolling circle amplification-terahertz metamaterial biosensor for parallel detection of multiple pathogenic bacteria and its detection method
  • Rolling circle amplification-terahertz metamaterial biosensor for parallel detection of multiple pathogenic bacteria and its detection method
  • Rolling circle amplification-terahertz metamaterial biosensor for parallel detection of multiple pathogenic bacteria and its detection method

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Embodiment Construction

[0030] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0031] Rolling circle amplification-terahertz metamaterial biosensor for parallel detection of multiple pathogens, including terahertz metamaterials, capture probes and polystyrene slides coated in terahertz metamaterial reaction zones to identify multiple pathogens, The polystyrene glass slide is covered on the upper layer of the terahertz metamaterial and forms a liquid film cavity for the liquid phase amplification reaction, and the height of the liquid film cavity is about 50 μm; the structure of the terahertz metamaterial is as follows figure 1 As shown, it is compose...

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Abstract

The invention discloses an RCA (rolling circle amplification)-terahertz metamaterial biosensor for parallel detection of multiple pathogenic bacteria, and a detection method of the biosensor. The biosensor comprises a terahertz metamaterial, capturing probes used for recognizing different pathogenic bacteria and coated with reaction zones made of the terahertz metamaterial, and a polystyrene slide; the upper layer of the terahertz metamaterial is covered with the polystyrene slide and forms a liquid film cavity used for liquid phase amplified reaction; the coated capturing probes of the RCA-terahertz metamaterial biosensor are hybridized with RCA annular templates to realize in-situ replication amplification. A stable and reliable result can be obtained with the method, and the parallel detection of six clinical common pathogenic bacteria can be performed within 3 hours.

Description

technical field [0001] The invention belongs to the detection field, and in particular relates to a rolling circle amplification-terahertz metamaterial biosensor for parallel detection of various pathogenic bacteria, and also relates to a method for parallel detection of various pathogenic bacteria by using the biosensor. Background technique [0002] Rapid and accurate detection of the species of pathogenic bacteria is crucial for the diagnosis and follow-up treatment of clinical infectious diseases. At present, in clinical microbiology, diagnostic methods based on the enzymatic and biochemical reaction characteristics of bacteria after culture are still widely used. Although this classic method is accurate, it often takes a long time, usually after the bacteria are isolated and cultured. It takes 7 days to conduct complex biochemical reaction experiments to accurately obtain the type of pathogenic bacteria. Although the matrix-assisted laser desorption ionization-time-of-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/34C12Q1/68C12Q1/14C12Q1/10C12Q1/04
CPCC12Q1/682C12Q1/6825C12Q2531/125C12Q2543/101C12Q2537/162C12Q2565/607Y02A50/30
Inventor 杨翔府伟灵黄庆罗阳刘跃平余抒徐含青赵祥刘羽杨柯黄娇祺
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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