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Disease-resistant variety breeding method of wild ganoderma lucidum

A disease-resistant variety, the technology of red lucidum, applied in the field of wild red lucidum disease-resistant variety breeding, can solve the problems of weak disease resistance and high temperature resistance, yield and quality decline, and lack of active ingredients, etc., to achieve comprehensive disease resistance, The effect of high active ingredients and strong disease resistance

Inactive Publication Date: 2016-04-20
ZHENJIANG SHENGHONG LANDSCAPE PLANT
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0002] Most of the parent species of Ganoderma lucidum in my country are imported from Japan and South Korea. After years of repeated cultivation, they have seriously degraded. The active ingredients of artificially cultivated Ganoderma lucidum are less than 1 / 3 of those of wild Ganoderma lucidum, and the main active ingredients such as polysaccharides and triterpenes of Ganoderma lucidum are constantly missing. And the ability to resist diseases and high temperatures is weak, resulting in a decline in yield and quality year by year

Method used

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  • Disease-resistant variety breeding method of wild ganoderma lucidum

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Experimental program
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Effect test

Embodiment 1

[0014] A method for breeding disease-resistant varieties of wild red lucidum, comprising the following steps:

[0015] S1. Cultivation of the starting strain: directly extract young somatic cells from the growing wild red lucidum vegetative body to induce callus, and then gradually cultivate the mycelium, and select white and strong mycelium as the starting strain; In this embodiment, wild red lucidum with bright color, no pests and diseases, and robustness is selected for somatic cell extraction;

[0016] S2. Inoculate the starting bacterial species in the PDA medium containing Trichoderma viride spores, name this medium A1, and cultivate it in a 25°C incubator, select the vigorous fruiting body for tissue isolation, and isolate the bacterial strain Small-scale cultivation was carried out to screen out the two strains with the best mycelium growth;

[0017] S3. Culture the above two bacterial strains into mycelia in cultivation bottles respectively, and accept 0.62 len / day g...

Embodiment 2

[0019] A method for breeding disease-resistant varieties of wild red lucidum, comprising the following steps:

[0020] S1. Cultivation of the starting strain: directly extract young somatic cells from the growing wild red lucidum vegetative body to induce callus, and then gradually cultivate the mycelium, and select white and strong mycelium as the starting strain; In this embodiment, wild red lucidum with bright color, no pests and diseases, and robustness is selected for somatic cell extraction;

[0021] S2. Inoculate the starting strains in PDA medium containing Trichoderma viride spores, and cultivate them in a 33°C incubator, select the vigorous fruiting bodies for tissue isolation, and carry out small-scale cultivation of the isolated strains, and screen out the strains The two strains with the best silk growth;

[0022] S3. Culture the above two bacterial strains into mycelia in cultivation bottles respectively, and accept 0.62 len / day gamma ray irradiation for 10 days...

Embodiment 3

[0024] A method for breeding disease-resistant varieties of wild red lucidum, comprising the following steps:

[0025] S1. Cultivation of the starting strain: directly extract young somatic cells from the growing wild red lucidum vegetative body to induce callus, and then gradually cultivate the mycelium, and select white and strong mycelium as the starting strain; In this embodiment, wild red lucidum with bright color, no pests and diseases, and robustness is selected for somatic cell extraction;

[0026] S2, inoculate the starting strains in the PDA medium containing Trichoderma viride spores, Penicillium, Paecilomyces and Aspergillus spores, name this medium A2, and cultivate it in a 25°C incubator, and select the robust ones The fruiting body is separated from the tissue, and the isolated strains are cultivated in a small test, and the two strains with the best mycelial growth conditions are screened out;

[0027] S3. Culture the above two bacterial strains into mycelia i...

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Abstract

The invention relates to a disease-resistant variety breeding method of wild ganoderma lucidum. An original strain is a mycelium cultured after wild ganoderma lucidum vegetative mass cells induce calluses, the original strain is inoculated in a PDA culture medium containing trichoderma viride spores and cultured in a constant-temperature box which is 25 DEG C, strong fruiting bodies are selected for tissue separation, separated strains are subjected to laboratory-scale cultivation, two best strains are screened out, the two strains are cultured into mycelia in cultivation bottles respectively and irradiated by gamma-ray with the radiation intensity of 0.58-0.76 r / day for 10 days, the strains with higher mycelia concentration degree are selected for rejuvenated culture, different strains are separated and purified, and the optimal strain is selected according to mycelia growth conditions to serve as a new disease-resistant variety. The ganoderma lucidum variety bred through the method is high in trichoderma viride resistance, and the problems that the ganoderma lucidum yield is low and the quality of the ganoderma lucidum is poor because of trichoderma viride can be prevented.

Description

technical field [0001] The invention relates to the field of ganoderma lucidum breeding, in particular to a method for breeding disease-resistant varieties of wild red lucidum. Background technique [0002] Most of the parent species of Ganoderma lucidum in my country are imported from Japan and South Korea. After years of repeated cultivation, they have seriously degraded. The active ingredients of artificially cultivated Ganoderma lucidum are less than 1 / 3 of those of wild Ganoderma lucidum, and the main active ingredients such as polysaccharides and triterpenes of Ganoderma lucidum are constantly missing. And the ability to resist diseases and high temperatures is weak, resulting in a decline in yield and quality year by year. Therefore, the breeding of disease-resistant varieties with wild Ganoderma lucidum native to my country as the mother species has become the top priority of Ganoderma lucidum breeding. Contents of the invention [0003] In order to solve the above...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H15/00
CPCA01H1/06A01H15/00
Inventor 李谊忠王承宏
Owner ZHENJIANG SHENGHONG LANDSCAPE PLANT
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