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RXDP2 gene SNP marking composition related to sheep horn phenotype and application thereof

A RXFP2-SNP1, sheep horn technology, applied in the field of genetic biology, can solve the problem of species disappearance

Active Publication Date: 2016-04-13
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Local breeds are usually welcomed by farmers and consumers in livestock production, but in recent years sheep hybridization has led to the disappearance of some local breeds, and the protection of local breeds is imminent
But so far there is no report that the SNP site of this gene is related to Mongolian sheep, Tibetan sheep and small-tailed Han sheep horn type

Method used

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  • RXDP2 gene SNP marking composition related to sheep horn phenotype and application thereof
  • RXDP2 gene SNP marking composition related to sheep horn phenotype and application thereof
  • RXDP2 gene SNP marking composition related to sheep horn phenotype and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Collect 5ml of anterior vena cava blood of the sheep to be tested, and extract DNA by traditional phenol imitation method.

[0036] (2) Use RXFP2 forward primer and reverse primer to perform PCR amplification: the PCR reaction system is as shown in Table 2, and the amplification program is as shown in Table 3:

[0037] Table 2

[0038] PCR reaction components

10μl system

5×GoldStar Taq PCR Buffer (cwbiotech)

2.0μl

dNTP (2.5mM each, Takara)

0.8μl

Primer Mix

2.0μl

GoldStar Taq DNA Polymerase (5U / μl, cwbiotech)

0.15μl

dna

1.0μl

wxya 2 o

4.2μl

Total

10μl

[0039] table 3

[0040]

[0041] (3) Digest the amplified PCR product, the digestion system is shown in Table 4, and the digestion conditions are 37° C., 60 min; 75° C., 15 min.

[0042] Table 4

[0043] components

content

PCR product

10μl

SAP (1U / μl)

3.3μl

ExoI (20U / μl)

...

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Abstract

The invention relates to an SNP marking composition related to a sheep horn phenotype. The SNP marking composition is located on a sheep RXDP2 gene and comprises RXDP2-SNP1 and RXDP2-SNP2; according to the annotation (XM_015097982) of a database latest version RXDP2 of the National Center for Biotechnology Information (NCBI), C->T mutation at the chr10: 29439053 (1999) locus serves as the RXDP2-SNP1, and C->T mutation at the chr10: 29439007 (1957) locus serves as the RXDP2-SNP2. By means of the SNP marking composition, the phenotypic characteristics of sheep horns can be identified, and therefore animal production and local variety protection can be guided; particularly, pure line Tibetan sheep can be identified by means of the SNP marking composition. Meanwhile, the loci have the greater scientific research value, and some genetic evolutionary laws can be researched through the obvious horn phenotype model.

Description

technical field [0001] The invention belongs to the field of genetic biology, and in particular relates to the RXFP2 gene SNP marker combination and application thereof related to the sheep horn phenotype. Background technique [0002] Sheep are the main animals raised in animal husbandry in my country, and their morphological characteristics are full body and dense hair. The head is shorter, and male sheep usually have spiral-shaped pointed horns. The main local breeds of sheep in my country include Mongolian sheep and Tibetan sheep. Among them, Mongolian sheep are mainly produced in the Inner Mongolia Autonomous Region, including small-tailed Han sheep, Hu sheep, Tan sheep, Ujimqin sheep, Bayinbulak sheep, etc. The horn type of Mongolian sheep is characterized by typical short and medium horns or no horns. Corner, the extension direction of the corner is to rotate close to the face. The types of Tibetan sheep mainly include grassland-type Tibetan sheep, Euler Tibetan sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 储明星潘章源刘秋月狄冉胡文萍王翔宇曹晓涵
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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