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Preparation method and application of crocodile bone collagen peptide powder

A collagen peptide and bone collagen technology, which is applied in skin care preparations, medical preparations containing active ingredients, applications, etc., can solve the problems of insignificant biological effects, inability to hydrolyze crocodile bone collagen, etc. Good social value and economic value, significant inhibitory effect, significant effect

Active Publication Date: 2016-04-06
GUANGZHOU TUOLONG BIOTECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the raw material is crocodile bone, the texture of the ingredients is relatively special, ordinary proteases cannot completely hydrolyze crocodile bone collagen, and the biological effect of the hydrolyzed product is not significant

Method used

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  • Preparation method and application of crocodile bone collagen peptide powder
  • Preparation method and application of crocodile bone collagen peptide powder
  • Preparation method and application of crocodile bone collagen peptide powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Effects of different proteases on the hydrolysis of crocodile bone glue

[0028] Take fresh crocodile bones, remove excess meat and fat, wash, chop into small pieces, add appropriate amount of distilled water, the ratio of the weight of crocodile bones to the volume of water added is 1:3, cook in a pressure cooker (GI54, Zealway, USA) (Pressure about 0.4MPa) After 30 minutes, cool and concentrate to 50% of the total volume of the solution to obtain crocodile bone glue. Get 6 parts of equal amounts of crocodile bone glue, put them into 6 beakers respectively, add 1% neutral protease based on the weight of bone glue in each beaker (the neutral protease from Bacillus subtilis, Zaozhuang Jienuo Bioenzyme Co., Ltd. ), acid protease (acid protease from A. ), compound protease (CN compound protease, Wuxi Jieren Biotechnology Co., Ltd.), and trypsin (Wuxi Jieren Biotechnology Co., Ltd.). The specific enzymatic hydrolysis conditions are shown in Table 1. After 3 hours, heat ...

Embodiment 2

[0038] Embodiment 2: Preparation of crocodile bone collagen peptide powder

[0039] Take fresh crocodile bones, remove excess meat and fat, wash, chop into small pieces, add appropriate amount of distilled water (the weight of crocodile bones to the volume ratio of added water is 1:3) and cook in a pressure cooker (GI54, Zealway, USA) (Pressure about 0.4MPa) After 30 minutes, cool and concentrate to 50% of the total volume of the solution to obtain crocodile bone glue. Add 1.6% papain (Shanghai Sangong) based on the weight of crocodile bone glue, hydrolyze at 55° C. for 4 hours, heat and boil for 10 minutes to inactivate the enzyme. Then centrifuge at 4500 rpm for 15 minutes at room temperature, take the supernatant, and freeze-dry to obtain powdered crocodile collagen peptide.

[0040] The aqueous solution of crocodile collagen peptide powder has a maximum absorption peak at around 220nm after being scanned by full-wavelength ultraviolet light, which is the characteristic ...

Embodiment 3

[0041] Embodiment 3: the antioxidant effect of the crocodile collagen peptide powder of the present application

[0042] 1. The crocodile collagen peptide powder of the present application is effective against DPPH, . Effect of OH scavenging ability and reducing power 1) Effect on DPPH scavenging ability:

[0043] The aqueous solution (0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0, 2.2mg / mL) of the different concentrations of the crocodile collagen peptide powder prepared in Example 2 of 2mL was placed in a 15mL test tube, and 2mL of 0.1mol / LDPPH solution (prepared with 95% ethanol), mix well, react at room temperature for 30min, meanwhile use 95% ethanol as blank control, measure absorbance value (A) at 517nm wavelength, calculate DPPH . clearance rate. Clearance rate = (A 样品 -A 空白 ) / A 空白 ×100%.

[0044] 2 pairs . Effect of OH scavenging capacity:

[0045] The salicylic acid method was used to determine the scavenging of hydroxyl radicals ( . OH) capability. Add 1mL...

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Abstract

The invention relates to a preparation method of crocodile bone collagen peptide powder. The method includes the steps that A, crocodile bones with muscles and fat removed are cooked in solvent under high pressure, concentration is conducted, and crocodile bone collagen peptide is obtained; B, the obtained crocodile bone collagen peptide is processed through protease, and enzymatic hydrolysate is obtained; C, centrifugal separation is conducted on the enzymatic hydrolysate, supernatant is taken and dried, and crocodile bone collagen peptide powder is obtained. The preparation method is simple, easy to implement and suitable for large-scale production, the additional value for crocodile product development can be improved, resource waste is avoided, environment pollution can be reduced, and good economic benefits and social benefits are achieved.

Description

technical field [0001] The present application relates to the field of biological products, more specifically, to a preparation method and application of crocodile collagen peptide powder. Background technique [0002] Collagen is the most abundant type of protein in the human body or other animals. It mainly exists in bone, skin, tendon, blood vessel and other tissues. As a very important protein in connective tissue, it plays the role of supporting organs and protecting the body. As a natural polymer compound, collagen has biocompatibility and degradability unmatched by synthetic polymer materials, so it is widely used in medicine, food, cosmetics and other fields. [0003] Collagen peptide is a new type of collagen product, which is prepared from collagen or collagen-rich substances. It has unique physiological functions not possessed by procollagen or constituent amino acids. It can be completely dissolved in water. The viscosity of the aqueous solution is low and the m...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K8/65A61Q19/02A23L33/18
CPCA23V2002/00A61K8/65A61K2800/782A61Q19/02C12P21/06A23V2200/02A23V2200/318
Inventor 陈清西曹兴梅李华亮董欣熊佑熊
Owner GUANGZHOU TUOLONG BIOTECH DEV
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