Chicken growth performance breeding molecular marker GHR (growth hormone receptor) gene and application thereof

Abstract

The invention relates to the gene engineering technology for molecular breeding of animals, in particular to a chicken growth performance breeding molecular marker GHR (growth hormone receptor) gene and an application technology thereof. Wuding chickens and Daweishan mini-chickens specific to the Yunnan Province are used as research materials, an SNP (single nucleotide polymorphism) site related to the growth performance of the Daweishan mini-chickens and the Wuding chickens is screened out and identified with a target area deep re-sequencing method in combination with bioinformatics analysis, molecular marking is performed, a 571A / G mutation site (the 75630 site of the GHR gene) significantly related to the weight is screened out of an intron 5 of the GHR gene and used as a molecular marker for assisted selection for chicken breeding, and the important science reference is provided for breeding of local chickens in the Yunnan Province.

Description

technical field [0001] The invention relates to the genetic engineering technology of animal molecular breeding, in particular to a chicken growth performance selective breeding molecular marker GHR gene and its application technology. Background technique [0002] Growth hormone receptor (GHR) is located on the GH-GHR-IGF axis, and regulates the expression of IGFs by mediating growth hormone (GH), thus playing an important role in regulating growth and development in vivo. The combination of GH and GHR can regulate animal growth. It has the functions of regulating nutrient distribution, promoting protein synthesis, reducing fat deposition, promoting skeletal muscle growth, increasing animal growth speed and feed remuneration, and participating in animal immunity, reproduction, lactation and other physiological functions and metabolism. adjustment. [0003] GHR is necessary for normal development of animals. GH plays an important role in promoting metabolic processes such ...

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Problems solved by technology

[0004] In the study of the GHR gene, human LTD dwarfism and sex-linked dwarf chickens are two well-studied examples of slow growth and short stature caused by GHR gene mutations. The liver lacks effective GHR, or GHR gene mutations, The effective combination of GH and GHR is deficient, leading to the interruption of the growth axis pathway, and finally leads to the low level of IGF-1. Even if the plasma GH concentration is significantly higher than that of normal chickens, the growth is still hindered, so the body size of SLD chickens is significantly lower than that of normal chickens individual

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