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Beta-ecdysterone-rich Cyanotis arachnoids extraction and purification method

A technology of ecdysterone and purification method, which is applied in the field of extraction and purification of dew grass rich in β-ecdysterone, which can solve the problems of inconspicuous drug effect and low content of β-ecdysterone

Inactive Publication Date: 2016-03-30
JIANGSU CAREFREE PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, dew grass preparations have been launched in my country, but due to the limitations of existing extraction and separation techniques, the content of β-ecdysterone is not high, resulting in insignificant efficacy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Select dew herbal medicinal materials with qualified quality, remove impurities, add to the extraction tank, add water with a volume to mass ratio of 10, heat and reflux for extraction for 1.5 hours, concentrate the filtrate under reduced pressure, add ethanol to make the alcohol content reach 50%, and the supernatant Concentrate under reduced pressure, put the concentrated solution on a HPD100 macroporous resin adsorption column, eluted with water and 5% ethanol respectively for a single volume to remove impurities, and then eluted with 6 times the amount of 40% eluent, collected the eluent, and washed Deliquified and concentrated, extracted twice with an equal amount of ethyl acetate, and retained the ethyl acetate layer; concentrated under reduced pressure to collect the ethyl acetate, dried, separated and purified on a silica gel chromatography column, collected fractions according to the TLC tracking results, and concentrated under reduced pressure Obtain the dried ...

Embodiment 2

[0020] Select quality dew herbal medicinal materials, remove impurities, add to the extraction tank, add water with a volume to mass ratio of 12, heat and reflux for extraction for 1.5 hours, extract twice, combine the filtrate, concentrate the filtrate under reduced pressure, add ethanol to make the alcohol content reach 60%, the supernatant is concentrated under reduced pressure, the concentrated solution is put on the HPD100 macroporous resin adsorption column, and the single volume is eluted with water and 5% ethanol respectively for impurity removal, and then the 50% eluent with a volume mass ratio of 6 Elution, collect the eluent, concentrate the eluent, extract twice with an equal amount of ethyl acetate, and keep the ethyl acetate layer; concentrate under reduced pressure to collect the ethyl acetate, dry, and separate and purify on a silica gel chromatography column, according to Fractions were collected from TLC tracking results, concentrated under reduced pressure to...

Embodiment 3

[0022] Select quality dew herbal medicinal materials, remove impurities, add to the extraction tank, add water with a volume to mass ratio of 14, heat and reflux for extraction for 1.5 hours, extract three times, combine the filtrate, concentrate the filtrate under reduced pressure, add ethanol to make the alcohol content reach 70%, the supernatant was concentrated under reduced pressure, and the concentrated solution was put on a HPD100 macroporous resin adsorption column, and the single volume was eluted with water and 5% ethanol respectively for impurity removal, and then washed with 70% eluent with a volume to mass ratio of 6 Eluate, collect the eluent, concentrate the eluent, extract 3 times with an equal amount of ethyl acetate, and keep the ethyl acetate layer; concentrate under reduced pressure to collect the ethyl acetate, dry, and separate and purify on a silica gel chromatography column, according to TLC Follow up the results to collect fractions, concentrate under r...

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PUM

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Abstract

The invention discloses a beta-ecdysterone-rich Cyanotis arachnoids extraction and purification method. The content of beta-ecdysterone in an extract product is 50-100%, and the extract product is prepared from Cyanotis arachnoids through the steps of water refluxing extraction, alcohol precipitation, resin adsorption and recrystallization. The content of the beta-ecdysterone in the Cyanotis arachnoids extract product obtained through adopting the extraction method is greatly improved, and the purity of the extract product reaches 90% or above. The above preparation method is suitable for industrial large-scale production, and is cheap.

Description

field of invention [0001] The invention belongs to the technical field of extraction of plant effective components, and in particular relates to an extraction and purification method of dew grass rich in β-ecdysterone. Background of the invention [0002] Dew grass (Cyanotis arachnoides C.B. Clarke) is a perennial herbaceous plant of the genus Pyrethriaceae. The aliases include pearl dew grass, cockscomb ginseng, Anemarrhena, spider hair and blue ear grass, etc. Dew grass is used as medicine from its roots. Numbness, lumbago and leg pain, nephritis edema, persistent deficiency and other symptoms, it can also be used externally to treat ankle pain, eczema, cuts, etc. In my country, dew grass is mainly distributed in Yunnan, Guangdong, Guangxi, Guizhou, Taiwan, Fujian and other regions, and it is also distributed in India, Sri Lanka, Vietnam, Laos, and Cambodia abroad. β-ecdysterone is extracted from Dewweed, which mainly contains a natural steroidal compound with insect mol...

Claims

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Application Information

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IPC IPC(8): C07J9/00
Inventor 秦引林殷连珍
Owner JIANGSU CAREFREE PHARM CO LTD
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