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Preparation method of skin cell damage repairing reagent

A technology for damage repair and skin cell application in the field of preparation of skin cell damage repair reagents, which can solve the problems of long time for cells to crawl out, affect research progress, and long separation time, and achieve low immunogenicity, improved efficacy, and biological activity strong effect

Inactive Publication Date: 2016-03-30
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The digestion method to separate umbilical cord mesenchymal stem cells has the problems of long separation time and high cost
2. Although the climbing method does not use collagenase to reduce the cost, the time for the cells to climb out is too long. Generally, a small number of cells can climb out after 13-15 days, which affects the progress of the experimental research.

Method used

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  • Preparation method of skin cell damage repairing reagent
  • Preparation method of skin cell damage repairing reagent
  • Preparation method of skin cell damage repairing reagent

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Embodiment Construction

[0037] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0038] The invention provides a preparation method of a skin cell damage repair reagent, the method is composed of the following steps:

[0039] ①Take the umbilical cord of a full-term healthy fetus, and use colloidal gold and fluorescent quantitative PCR to detect umbilical cord serum HbsAg, anti-HCV, HCV-RNA, anti-HDV, anti-HEV, anti-HIV-1 / 2, HIV-1 -RNA, CMV-DNA, umbilical cords with negative test results can only be used;

[0040] ② Take the umbilical cord out of the collection bottle, place it in a sterile plate, and cut it into small pieces of about 5 cm with sterile surgical scissors;

[0041] ③Immerse the umbilical cord segment in a sterile beaker, and repeatedly wash the blood remaining in t...

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Abstract

The invention relates to a preparation method of a skin cell damage repairing reagent; P4-generation umbilical cord mesenchymal stem cells have a large number of cells, phenotypes are stable, a lot of mixed cytokines can be secreted, and the requirements of mass production of the cell damage repairing reagent can be met, so that the purpose that the cell damage repairing reagent is applied in clinical treatment of skin damage repairing is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and is mainly applied to the separation and cultivation of adherent cells, in particular to a preparation method of a reagent for repairing skin cell damage. Background technique [0002] Mesenchymal stem cells (mesenchymal stem cells, MSCs) are a special type of stem cells derived from the mesoderm and ectoderm in the early stages of development. They are classified as pluripotent stem cells. Under specific induction conditions in vivo or in vitro, MSCs can differentiate into adipose tissue. Cells, bone cells, chondrocytes, nerve cells, liver cells, cardiomyocytes, endothelial cells and other tissue cells still have multi-directional differentiation potential after continuous subculture and cryopreservation, and can be used as ideal seed cells for aging and disease Due to its multi-directional differentiation potential, it can be widely used in the treatment of various diseases such as liver fibrosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61P17/00A61K31/728
CPCA61K35/28A61K31/728A61K47/36A61K2300/00
Inventor 楼敏铭王宏伟马洁赵刚韩阳光
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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