Iron oxidase diagnostic kit

Abstract

The invention belongs to the technical field of biochemical analysis, and discloses an iron oxidase diagnostic kit. The iron oxidase diagnostic kit comprises components which are prepared from, by mass, 0.3%-1% of sodium acetate solution, 0.05%-5% of N,N-dimethyl-p-phenylenediamine-hydrochloride, 3.4%-11% of glacial acetic acid, 0.5%-1.1% of thiourea, 0.31%-3% of Fe(NH4)2(SO4)2.6H2O and 87%-92% of sterilization refined water. According to the iron oxidase diagnostic kit, on the basis that an amine compound has the property of oxidation relative to divalent iron ions, a biochemical measurement method of taking divalent iron, p-phenylenediamine and the like as substrates is established, diagnosis of iron oxidase is simple, convenient, rapid and good in repeatability, and the iron oxidase diagnostic kit is suitable for automated analysis.

Description

technical field [0001] The invention belongs to the technical field of biochemical analysis, in particular to a ferrous oxidase diagnostic kit. Background technique [0002] Ferrous oxidase is a protein synthesized by the liver. Studies suggest that its main function is to catalyze ferrous iron into ferric iron, and use iron to incorporate iron transporters to promote iron transport. Ferrous oxidase is a carrier of biogenic amine oxidase and copper. At the same time, ferrous oxidase is an acute phase reaction protein, and the level of ferrous oxidase in plasma increases significantly when infection and inflammation occur; the increase of ferrous oxidase is more obvious in high-risk pregnancy, and its determination is also used for monitoring high-risk pregnancy. Another role of ferrous oxidase, that is, its antioxidant effect has been paid more and more attention. [0003] Wilson's disease, also known as hepatolenticular degeneration, is an autosomal recessive genetic dise...

AI Technical Summary

Problems solved by technology

The enzyme-catalyzed method is non-specific for ferrous oxidase, and free ferrous ions will also affect the results, resulting in errors in the results; the immunological method is fast and accurate, but requires a large amount of serum and requires high purity. higher, higher cost

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