Specific gene of brown atrichopogon and molecular identification method of brown atrichopogon

A specific gene and molecular identification technology, applied in the field of species identification, can solve problems such as difficulty, long cycle, and cumbersome operation steps, and achieve the effect of shortening identification time and ensuring accuracy and reliability

Active Publication Date: 2016-03-23
FUJIAN INT TRAVEL HEALTH CARE CENT
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Problems solved by technology

At present, the identification of midges mainly depends on the identification of morphological characteristics by experienced taxonomic experts. It is very difficult to identify specimens with close relatives and incomplete morphological characteristics; moreover, the morphological identification of blood-sucking midges needs to pass Making slide specimens to complete, the operation steps are cumbersome, the cycle is long, and the difficulty is great. Therefore, it is urgent to find a new method to make up for the shortcomings of the traditional classification method

Method used

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  • Specific gene of brown atrichopogon and molecular identification method of brown atrichopogon
  • Specific gene of brown atrichopogon and molecular identification method of brown atrichopogon
  • Specific gene of brown atrichopogon and molecular identification method of brown atrichopogon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Acquisition of 18SrDNA gene sequence of Atrichopogon subfusculus

[0025] 1. Acquisition of the specimens of the subpalatin midge

[0026] The subpalatinous midges were collected from the field, and they were killed by freezing and stored in 95% ethanol directly. After the specimens are made into slides, they are identified by authoritative experts to ensure the accuracy of the identification results. Before making specimens, pick the end of the thorax and the first few segments of the abdomen of the sub-brown midge and place them in 95% alcohol for DNA extraction.

[0027] 2. DNA template preparation

[0028] Using the small insect DNA template preparation method to extract the DNA of the subpalatin midge (Edited by Wen Lizhang. Introduction to Entomological Research Methods and Techniques [M]. Beijing: Science Press, 2010.pp278-286), the extracted DNA samples are stored in Standby at -20°C.

[0029] 3. Primer synthesis

[0030] The primers used in this ...

Embodiment 2

[0045] The identification of embodiment 2 unknown naked midges

[0046] 1. Collection and preservation of specimens

[0047] The collected specimens were collected by waving nets or lamp lures, and the collected specimens were killed by freezing and stored in 95% ethanol directly.

[0048] 2. DNA template preparation

[0049] Under a dissecting mirror or a stereo microscope, a female nude midge was randomly selected from the collected midge specimens, and the DNA of the nude midge was extracted by using the small insect DNA template preparation method (Wen Lizhang, editor-in-chief. Entomological research methods and techniques Introduction [M]. Beijing: Science Press, 2010.pp278-286), the extracted DNA samples were stored at -20°C for future use, and each sample had a number.

[0050] 3. Acquisition of target gene sequence

[0051] 3.1 Primer synthesis

[0052] The obtained unknown naked midge DNA was used as a template to synthesize primers, and the sequences of the prime...

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Abstract

The invention discloses a specific gene of brown atrichopogon and a molecular identification method of the brown atrichopogon. The specific gene of brown atrichopogon and the molecular identification method of the brown atrichopogon are characterized in that a molecular biology method is adopted, the gene sequence 18S rDNA of the atrichopogon is obtained, and through comparison of gene sequence similarity, species identification is conducted on the atrichopogon. By means of the molecular identification method of the brown atrichopogon, it is facilitated to achieve accurate and rapid identification of the brown atrichopogon.

Description

technical field [0001] The invention relates to the field of species identification, in particular to a specific gene sequence of the subbrown midge and a molecular identification method thereof. Background technique [0002] Midges are tiny insects in the order Diptera, commonly known as small bites. At present, the identification of midges mainly depends on the identification of morphological characteristics by experienced taxonomic experts. It is very difficult to identify specimens with close relatives and incomplete morphological characteristics; moreover, the morphological identification of blood-sucking midges needs to pass The production of slide specimens is cumbersome, long-term, and difficult. Therefore, it is urgent to find a new method to make up for the shortcomings of traditional classification methods. [0003] Molecular identification technology is based on the analysis of genetic material DNA sequence to clarify the differences between species, and quickly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888
Inventor 杨美琼黄恩炯王宇平高博张建庆王飞鹏
Owner FUJIAN INT TRAVEL HEALTH CARE CENT
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