Eurotium amstelodami and application thereof
A kind of technology of saccharomyces and its application, which is applied in the field of syringomyces amsterdam in producing flower and fruit aroma substances, which can solve the problem of less research on aroma-producing substances, and achieve easy cultivation and preservation and industrial production, Low production cost, high tolerance effect
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[0029] 1. Isolation method of Eurotium amstelodami FBKL3.0013 strain:
[0030] Weigh 10g of Daqu sample from a distillery in Guizhou, put it into a Erlenmeyer flask filled with 90ml of sterile water and glass beads, and shake for about 30min. Take 1ml of the sample suspension for 10-fold gradient dilution, respectively take 1mL of the 10-4 and 10-5 dilutions of the sample suspension and spread it on the separation medium, culture it statically at 30°C for 5 days, and pick a single colony for further screening. Stored on slant medium.
[0031] 2. Strain identification method:
[0032] The isolated and purified strains were inoculated on CA medium and 20% Chase agar medium, cultivated in a 25°C incubator for 5-20 days, observed the morphological characteristics of the colonies, and stained with lactic acid carbolic wool blue staining solution to prepare slides. The morphological characteristics of mycelia and spores were observed under an optical microscope, and the morphologi...
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