Growth and differentiation culture medium for somatic embryo of pear tree

Abstract

A growth and differentiation culture medium for a somatic embryo of a pear tree is characterized by comprising 1 / 2 improved MS, 1.0-1.5 mg / L 6-BA, 0.5-1.0 mg / L 2,4-D, 2.0-3.0 mg / L KT, 6.0-7.0 g / L agar, 20 g / L saccharose and 2.0%-2.5% phytagel, wherein the pH (potential of hydrogen) of the culture medium ranges from 5.8 to 6.0. The pear tree difficult to propagate can propagate quickly through somatic embryogenesis on the basis of improvement of a basic culture medium and ingredients and adjustment of a culture condition, the survival rate of transplanted plants can be higher than 96%, seedlings grow robustly, the germplasm degeneration problem of good seedlings can be solved effectively, a large quantity of purified and rejuvenated nursery stocks can be provided for production, and an ideal receptor system is provided for breeding of new varieties during genetic transformation or mutation breeding of the pear tree.

Description

technical field [0001] The invention relates to a growth and differentiation medium for somatic embryos of isolated pear tree leaves, belonging to the field of botany. Background technique [0002] Pear trees belong to the genus Pyrus in the family Rosaceae, perennial deciduous fruit trees and trees, and are widely distributed around the world. There are about 35 native species in the world. They are wildly distributed in Europe, Asia and Africa. They are classified into two types: Oriental pear and Western pear. kind. China's pear cultivation area and output are second only to apples. [0003] Pear tree is a kind of tree species with high development value. At present, the propagation method usually adopted is cutting propagation, and this propagation method is susceptible to fungal diseases such as leaf spot. It is also vulnerable to aphids in spring and summer. The virus Seriously, the leaf mottling is easy to fall off, and the degree of improved variety is reduced; and...

AI Technical Summary

Problems solved by technology

[0003] Pear tree is a kind of tree species with high development value. At present, the propagation method usually adopted is cutting propagation, and this propagation method is susceptible to fungal diseases such as leaf spot. It is also vulnerable to aphids in spring and summer. The virus Seriously, the leaf mottling is easy to fall off, and the degree of improved variety is reduced; and the use of inferior seedlings leads to the loss of the inherent good characteristics of pear trees, which restricts the development and popularization of this tree species to a certain extent. Therefore, selection, breeding Excellent pear tree strains have become a very prominent production problem. Tissue culture rapid propagation is an effective way for the industrialization of improved pear tree varieties, and there are many related reports. Systematic technical research has no successful reports