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Method for inducing disease resistance of pepper system by taking phosphite as exciton

A phosphite and disease resistance technology, applied in horticultural methods, botanical equipment and methods, seed and rhizome treatment, etc., can solve problems such as limiting the in-depth study of disease prevention mechanism, and achieve short experimental cycle and experimental repeatability Good, easy to observe the effect of the experiment

Active Publication Date: 2016-03-23
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research shows that the lack of analysis of the molecular mechanism of phosphite in the defense response of pepper disease resistance limits our in-depth research on its disease prevention mechanism

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Prepare a phosphite solution according to the weight ratio of 1g of phosphorous acid: 1g of potassium hydroxide, first dissolve 1g of phosphorous acid in water, then dissolve 1g of potassium hydroxide on the basis, and dilute the above solution to 1000mL, and prepare the prepared phosphite The pH value is about 6.2, and it can be applied directly by adding Tween-20, which accounts for 2‰ of the total fraction; Taiwan pepper 106 and American Xiyuan pepper seeds are treated with 30% volume of sodium hypochlorite (Shanghai Sinopharm Cas#:7681-52-9) surface After disinfection, rinse with sterilized distilled water, scald the seeds in a 55°C water bath for 20 minutes, sow the seeds in an enamel tray (30*50cm) with two layers of gauze underneath, and then place them in a 28°C incubator. Cultivate for 5 days under dark and light-shielding conditions until long and thin pepper yellow seedlings grow, and then remove them from the light incubator and cultivate them for 2 days; Th...

Embodiment 2

[0019] Prepare a phosphorous acid solution according to the weight ratio of phosphorous acid 1g: potassium hydroxide 1g, first dissolve 1g of phosphorous acid with water, then dissolve 1g of potassium hydroxide on the basis, set the volume of the above solution to 1000mL, and prepare the prepared phosphite The pH value is 6.2, adding Tween-20 which accounts for 2‰ of the total fraction can be applied directly; the seeds of Red Sun and Spring Spicy No. 19 are surface-sterilized with 30% sodium hypochlorite, rinsed with sterilized distilled water, and placed in a water bath at 58°C After medium scalding for 20 minutes, sow the seeds in an enamel tray (30*50cm) with two layers of gauze underneath, and then culture them in a 28°C incubator under dark and dark conditions for 7 days until long and thin peppers grow. The etiolated seedlings were removed from the light incubator and cultivated for 3 days; the detection method of the efficacy of the phosphorous acid elicitor inducing th...

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Abstract

The present invention relates to a method for inducing disease resistance of a pepper system by taking phosphite as an exciton and an application. The method comprises the following steps: firstly, preparing the phosphite; secondly, producing pepper seedlings; and finally, detecting disease resistance defense reaction performance of a phosphite exciton induced pepper, treating the cultured pepper seedlings with the prepared phosphite in a spraying method, performing inducing treatment for 48 hours, then inoculating the etiolated seedlings with Phytophthora capsici Zoospore suspension liquid, and observing morbidity of the seedlings after 3-5 days to determine the effect of the liquid to induce plant disease resistance. By the method, the phosphite adapted to the field application can be prepared in a time and labor saving manner, meanwhile, disease resistance defense reaction activation is detected, so that the gap of understanding in studying disease resistance of pepper by using phosphite as the exciton to activate defense responses and to enhance disease resistance is filled up, and the method has a great significance.

Description

technical field [0001] The invention relates to the technical field of plant protection, and discloses a method for inducing disease resistance of capsicum system by using phosphite as an elicitor. Background technique [0002] Pepper blight is a devastating fungal disease that occurs worldwide. It was first discovered in New Mexico, USA in 1918. Phytophthora capsici caused by pepper blight Phytophthoracapsici Leonian was named by Leon Leonian in 1922, and it belongs to plant pathogenic oomycetes. Phytophthora capsici overwinters in diseased soil as oospores or chlamydospores, and can survive for several months or even longer. It has a wide range of hosts and mainly infects many important vegetable crops such as peppers, tomatoes, eggplants, cucumbers, and pumpkins. Capsicum blight can occur in both open fields and protected areas. The prevalence of the disease has caused serious losses of peppers. The general rate of diseased plants is 15-30%, and it can reach more than 80...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C1/00A01G7/00
CPCA01C1/00A01G7/00A01G22/00
Inventor 刘裴清陈庆河李本金翁启勇
Owner INST OF PLANT PROTECTION FAAS
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