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Technetium 99m-marked anti-aspergillus monoclonal antibody for detection of aspergillus, and preparation and application thereof

A technology of Aspergillus and Aspergillus single, applied in the field of technetium 99-labeled anti-Aspergillus monoclonal antibody Mab-WF-AF-1, which can solve the problem of different reports on detection sensitivity and specificity, and no standardized operating procedures and equipment Higher requirements and other issues, to achieve the effect of good in vitro stability, high labeling rate, and high sensitivity

Inactive Publication Date: 2016-03-16
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV +1
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Problems solved by technology

Although the sensitivity and specificity of the detection method based on PCR technology are relatively ideal, its disadvantages lie in the occurrence of contamination problems and false positive results, as well as the absence of standardized operating procedures, high requirements for equipment, unavoidable cross-reactions, and the sensitivity of detection. Reports of sex and specificity vary, and these issues limit its clinical application

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  • Technetium 99m-marked anti-aspergillus monoclonal antibody for detection of aspergillus, and preparation and application thereof
  • Technetium 99m-marked anti-aspergillus monoclonal antibody for detection of aspergillus, and preparation and application thereof
  • Technetium 99m-marked anti-aspergillus monoclonal antibody for detection of aspergillus, and preparation and application thereof

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Embodiment 1

[0027] A technetium-99-labeled anti-aspergizumab for the detection of Aspergillus fumigatus (Af), through the bifunctional linker NHS-MAG3 and the mouse anti-Aspergillus monoclonal antibody Mab-WF-AF-1 and 99m TCO 4 - join gets.

[0028] (1) Preparation of technetium-99-labeled anti-Aspergillus for detection of Aspergillus fumigatus:

[0029] Mouse anti-Aspergillus monoclonal antibody Mab-WF-AF-1 (purchased from AbDSerotec Company, MCA2576) was placed in a millipore ultrafiltration centrifuge tube, and after centrifugation to remove sodium azide, the protein obtained from 10 ug was dissolved in 50 ul. In 3M HEPES buffer solution (pH=8-8.5), add bifunctional linker NHS-MAG3 (according to WangY, LiuX, HnatowichDJ. An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with (99m) Tcatroomtemperature. NatProtoc2007; 2 (4): 972-978. Synthetic method), the molar ratio of the NHS group of the bifunctional linker NHS-MAG3 to the amino group on the monoc...

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Abstract

The invention provides a technetium 99m-marked anti-aspergillus monoclonal antibody for detection of aspergillus, and preparation and application thereof. The technetium 99-marked anti-aspergillus monoclonal antibody is characterized by being prepared through coupling of a difunctional coupling agent NHS-MAG3 with a mouse anti-aspergillus monoclonal antibody Mab-WF-AF-1 and <99m>TcO4<->. The prepared technetium 99m-marked anti-aspergillus monoclonal antibody can be used for qualitative and / or quantitative determination of aspergillus spores in the lung.

Description

technical field [0001] The invention relates to a technetium-99-labeled anti-aspergillus monoclonal antibody Mab-WF-AF-1, which can be used for detecting aspergillus in lungs. Background technique [0002] At present, the detection of Aspergillus is mainly based on morphology-based microscopic examination, culture and histopathological examination as the gold standard, but these techniques have low positive rate, time-consuming, easy to be contaminated, invasive inspection and difficult to identify species, etc. shortcoming. Serological and molecular biological methods are emerging technologies in the field of fungal infection detection. Serological examination mainly includes antigen antibody detection and metabolite detection. Since invasive Aspergillus infection is often accompanied by impaired immune function, the body may not be able to produce antibodies in an effective immune response. Therefore, clinically, antigen detection is mainly used, such as galactomannan (G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 金先桥党亚杰王桂芳
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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