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Method for rapidly detecting pesticide residue based on plant esterase

A plant esterase, fast technology, applied in the field of analytical chemistry, can solve the problems of difficulty in obtaining raw materials and high cost, and achieve the effects of high sensitivity and precision, simple operation, and strong anti-interference ability

Active Publication Date: 2016-03-16
CHONGQING UNIV
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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a new method for rapid detection of organophosphorus pesticides based on plant esterase, to solve the problems of difficult and high cost of raw materials for sensitive elements of biosensors, and to realize the detection of low-concentration organic pesticides. Qualitative and quantitative detection of phosphorus pesticides

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  • Method for rapidly detecting pesticide residue based on plant esterase
  • Method for rapidly detecting pesticide residue based on plant esterase
  • Method for rapidly detecting pesticide residue based on plant esterase

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Embodiment Construction

[0034]The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings.

[0035] 1. A method for rapid detection of organophosphorus pesticides based on plant esterase, The detection object is an organophosphorus pesticide (methyl parathion or malathion), which is detected by a biosensor based on plant esterase.

[0036] 1. Detection principle: see the detection principle figure 1 , in the hydrolysis equation (1), the substrate 1-naphthyl acetate is hydrolyzed by plant esterase to produce 1-naphthol and acetic acid (electroactive substances), thereby generating a current signal response, which will be inhibited with the addition of organophosphorus pesticides As the reaction proceeds, the electroactive products decrease, so the current signal decreases. With the difference in the amount of organophosphorus pesticide added, the magnitude of decrease in the current signal is different.

[0037] 2. Detection of ...

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Abstract

The invention provides a method for rapidly detecting pesticide residue based on plant esterase. The method comprises employing a three-electrode system, taking a biosensor as a working electrode, putting the three-electrode system in an organic phosphorus pesticide solution and performing inhibition for 6-15 min, then putting in a background solution and reacting for 3-8 min, selecting differential pulse voltammetry to establish a standard curve, and detecting the organic phosphorus pesticide content in a sample based on the standard curve. The biosensor is capable of realizing detection on a low-concentration organic phosphorus pesticide, the sensitivity is high, and the method possesses the following characteristics: the employed raw material is simple, cost is low, pretreatment is simple, the repeatability is high, and the application scope is extensive.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry, in particular to a method for rapidly detecting organophosphorus pesticides based on plant esterase. Background technique [0002] As one of the most widely used pesticides, organophosphorus pesticides enter the human body mainly through skin contact, breathing and direct consumption, and then circulate to various organs and tissues throughout the body through blood and lymph circulation. After entering the nervous system, organophosphorus pesticides combine with the active center of acetylcholinesterase to generate phosphorylated cholinesterase, which is difficult to hydrolyze and destroys the activity of cholinesterase, causing a large amount of acetylcholine to accumulate on nerve synapses , Interfering with the normal conduction of nerve impulses, can even lead to human death, and pose a great threat to human health. [0003] At present, the methods for the detection of pestic...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/327G01N27/48
Inventor 包静侯长军霍丹群杨眉
Owner CHONGQING UNIV
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