Establishment method for alcoholic fatty liver zebra fish model

Abstract

The invention discloses an establishment method for an alcoholic fatty liver zebra fish model. The establishment method includes the following steps that AB-series wild zebra fishes are domesticated in a laboratory, and the healthy adult zebra fishes are picked out to be evenly divided into 16 groups at random; a central combinational design method is adopted, and the ethyl alcohol concentration, the exposure time and the test cycle are designed; after the adult zebra fishes in all the groups are fed according to design conditions, the survival rates and average lipid droplet areas of the adult zebra fishes in the groups are calculated; the obtained 16 groups of survival rates and average lipid droplet areas are subjected to standard deviation standardization, survival rate standardized values and average lipid droplet area standardized values of all the groups are added and then divided by 2 to obtain the comprehensive grades of the adult zebra fishes in the groups; the comprehensive grades of all the groups are subjected to multivariate regression analysis and binomial fitting to obtain a regression equation, and the optimal modeling condition of the alcoholic fatty liver zebra fish model is determined according to the regression equation. The establishment method is low in cost and short in cycle, the alcoholic fatty liver pathomechanism can be deeply researched, and a novel treatment means is found for the alcoholic fatty liver.

Description

technical field [0001] The invention relates to the technical field of construction methods for model animal disease models, in particular to a construction method for a zebrafish model of alcoholic fatty liver disease. Background technique [0002] Alcoholic fatty liver is a pathological process of liver damage and excessive accumulation of lipids in liver cells caused by long-term heavy drinking. It is a type of alcoholic liver disease. The morphological changes and metabolic changes of hepatocytes in the process of alcoholic steatosis will bring more and more serious damage to the hepatocytes and affect the normal function of the liver. Compared with other types of fatty liver, alcoholic fatty liver is more likely to induce liver fibrosis and cirrhosis. 20% to 30% of fatty liver caused by ethanol may develop into cirrhosis and even lead to liver cancer. Although there are many theories and hypotheses about the pathogenesis of alcoholic fatty liver, none of them can cove...

AI Technical Summary

Problems solved by technology

However, at present, although some people use zebrafish embryos to construct alcoholic fatty liver models, the disadvantages are obvious: using zebrafish embryos as experimental animals, the disease model replicated is poorly similar to human diseases, and it cannot accurately induce diseases with similar diseases. The occurrence mechanism of human disease characteristics, so that the occurrence and development of alcoholic fatty liver cannot be effectively understood, so the embryonic model still cannot become a drug screening program at the overall animal level

Alcoholic fatty liver model research work using adult zebrafish remains unreported

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