Method for inducing artificial ovulation and spawning of fresh-water eels

Abstract

The present invention relates to a method for inducing artificial ovulation and spawning of fresh-water eels, wherein a feminization inducing material (e.g., 17beta -Estradiol) for inducing feminization is fed into a culturing bath in which brood fish as an object of artificial ovulation are cultured, for a predetermined period of time, and when the ovulation induction period has come, a flathead mullet pituitary extract (FPE) of flathead mullet, which are fish in rivers and broad streams, as an ovulation inducing agent is added in the culturing bath, thereby promoting artificial ovulation even without applying an administration manner using intraperitoneal injection. Preferably, according to the present invention, the ovulation and spawning of the brood fish can be induced by selectively setting the conditions of fresh water, brackish water, and seawater for culture water of the culturing bath, feeding a feminization inducing material for inducing complete feminization of the fresh-water eel brood fish into the culturing bath at the fresh water condition of the culture water, performing a pretreatment procedure for the feminization, setting the seawater condition of the culture water, and then feeding the ovulation inducing agent into the culture water.

Description

technical field [0001] The present invention relates to artificial ovulation and spawning induction method of freshwater eel, in more detail, relates to artificial ovulation and spawning induction method of freshwater eel, in the rearing tank of freshwater eel, mix appropriate amount of feminization-inducing substance and from freshwater eel Artificial ovulation is induced under the culture water conditions of the ovulation-inducing substance extracted from mullet, which is a similar anodal fish. Background technique [0002] In the case of freshwater eels belonging to the Japanese eel, in the artificial feeding environment, the synthetic ability of the pituitary gonadotropins (GTHs) is insufficient, resulting in underdeveloped gonads (Nagahania and Yamamoto, 1973), this glycoprotein sex Gonadotropins are essential for the formation and development of gonads in vertebrates including fish (Kumare et al., 1997; Ma et al., 2004). [0003] Therefore, regarding female freshwater...

AI Technical Summary

Problems solved by technology

[0005] In addition, in the case of repeated administration of salmon pituitary gland extract for artificial ovulation induction of freshwater eel broodstock, the dose per individual may be excessive or insufficient, and ultimately affect the egg quality of artificial ovulation.

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