Detection kit for human serum amyloid A protein

Abstract

The invention discloses a detection kit for human serum amyloid A protein. The detection kit is characterized by comprising a reagent R1 and a reagent R2, the reagent R1 is an appropriate buffer solution, and the reagent R2 is prepared by putting polystyrene latex particles sensitized by a human serum amyloid A protein antibody into the appropriate buffer solution. The detection kit has the advantages of being simple and fast in detection, high in sensitivity, good in accuracy, high in anti-jamming capability and low in production cost.

Description

technical field [0001] The invention belongs to the field of medical immunology, and relates to an immunodetection reagent, and further, the invention relates to a human serum amyloid A detection kit. Background technique [0002] Serum amyloid A (serumamyloidAprotein, SAA) is an acute phase response protein, which belongs to the heterogeneous protein in the apolipoprotein family, with a relative molecular weight of about 12,000. In the acute phase response, stimulated by IL-1, IL-6 and TNF, SAA is synthesized in the liver by activated macrophages and fibroblasts, which can increase to 100-1000 times the initial concentration, but the half-life Short, only about 50 minutes. Serum amyloid A is associated with high-density lipoprotein (HDL), which regulates HDL metabolism during inflammation. A particularly important property of serum amyloid A is that its degradation products can be deposited in different organs as amyloid A (AA) fibrils, which is a serious complication in ...

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Problems solved by technology

[0004] The existing SAA detection method is mainly enzyme-linked immunoassay. Due to the long cycle of enzyme-linked immunoassay, it cannot be used on automatic biochemical analyzers, and it is not suitable for rapid detection in emergency. The turbidimetric method is simple and economical, and can be used on the automatic biochemical analyzers in most hospitals, especially in emergency departments, where rapid quantitative detection can be achieved. After the reaction, aggregated particles are formed, and at a certain wavelength, the content of the analyte in the sample can be measured by measuring the turbidity generated by the aggregate

[0005] However, when the serum amyloid A antibody is coated on the latex, physical adsorption and chemical coupling methods can generally be used. The stability of the sensitized latex particles obtained by the physical adsorption method is worse than that of the chemical coupling method, and the antibody is easily released from the latex particles. come off; and the latex obtained by physical adsorption may be interfered by rheumatoid factor (RF) and heterophilic antibodies, and IgM and IgG type RF can directly combine with the Fc segment of the antibody coated on the latex, thereby Cause false-positive or false-negative test results to rise

Most of the chemical coupling methods currently used are random coupling methods, because randomly coupled antibodies are randomly coupled to different parts of the antibody, which will lead to the loss of binding force of the antibody; therefore, complete random coupling will lose most of the binding force of the antibody and increase Antibody dosage and production cost

At the same time, if the sensitized latex particles obtained by the above method are used in the detection kit, it will cause the defects of poor specificity, low accuracy, weak anti-interference ability or high production cost.

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