Sjogren syndrome specific autoantibody immunoblotting kit

Abstract

The invention discloses an immunoblotting kit for sjogren syndrome specific autoantibody detection. The kit includes: a blotting membrane strip placed in a reaction tank, an enzyme conjugate, a chromogenic reagent A, a chromogenic reagent B, a stop solution, a dilution solution, concentrated washing liquid, and a standard zone tape. The membrane strip is placed into the reaction tank to react with to-be-tested serum, if the to-be-tested serum contains a corresponding autoantibody, the autoantibody can combine with a corresponding antigen, then the enzyme conjugate and the chromogenic reagents are added, contrast with the standard zone tape is carried out to judge whether the serum contains the sjogren syndrome specific autoantibody: anti-SSA antibody (Ro), SSB (La), salivary gland protein 1 (SP1), carbonic anhydrase 6 (CA6), and parotid secretory protein (PSP). The existence of sjogren syndrome specific autoantibodies and the antibody species and quantity are of important clinical significance for sjogren syndrome diagnosis and differential diagnosis.

Description

technical field [0001] The invention relates to a Sjogren's syndrome specific autoantibody immunoblotting kit. Background technique [0002] Sjögren's syndrome is a common autoimmune disease that mainly involves salivary glands, lacrimal glands, and many other vital organs. Autoantibodies are important biomarkers in the diagnosis of Sjögren's syndrome. At present, the autoantibodies mainly used in the diagnosis of Sjögren's syndrome are SSA and SSB. However, due to the lack of high sensitivity of SSA and SSB, the diagnosis of a large number of patients with Sjögren's syndrome (especially early patients) still requires an invasive labial gland biopsy, which brings great benefits to the diagnosis and treatment of Sjogren's syndrome. obstacle. [0003] Currently, there is no western blot kit specifically for the detection of Sjogren's syndrome-specific autoantibodies. Most western blot strips cover autoantibodies related to various autoimmune diseases such as systemic lupus ...

AI Technical Summary

Problems solved by technology

Most western blot strips cover autoantibodies related to various autoimmune diseases such as systemic lupus erythematosus, scleroderma, dermatomyositis / polymyositis, lack of disease specificity, and cause waste of clinical resources

Anti-SP1, CA6, and PSP antibodies are new autoantibodies that can be used for the early diagnosis of Sjogren's syndrome, and there is no Western blot strip for detecting anti-SP1, CA6, and PSP autoantibodies