Screening method of composite microbial system for producing organic fertilizer

Abstract

The invention discloses a screening method of a composite microbial system for producing organic fertilizer. According to the method, samples are firstly collected from organic fertilizer which is completely decomposed or animal manure, then strains are separated from the samples through different culture media, dominant strains good in enzyme production effect are screened out through a filter paper collapse test and a transparent ring test and then are identified, purified and subjected to enrichment culture, the antagonism test is performed on the basis that the dominant strains are obtained, and the optimally-combined composite microbial system for producing organic fertilizer is obtained by analyzing the combination activity of bacterial strains. The composite microbial system for producing organic fertilizer is good in compatibility, the enzyme production capacity is obviously higher than that of single bacterial strains, and the activity of cellulase, protease and amylase of the composite microbial system for producing organic fertilizer reaches 193.5, 25.3 and 55.7 (U / ml) respectively. Thus, the obtained composite microbial system can effectively degrade straw, animal manure and other agricultural waste so that the organic fertilizer can be obtained, the environment can become clean, biological organic fertilizer can be processed, and sustainable development of agriculture is achieved.

Description

technical field [0001] The invention relates to a method for screening compound bacteria strains for producing organic fertilizer, which belongs to the technical field of agricultural microorganisms. Background technique [0002] With the intensive development of my country's agriculture and agricultural processing industry, a large amount of agricultural solid organic waste resources are wasted wantonly, such as rice, wheat, corn, rapeseed and other crop stalks are burned on the spot, livestock and poultry manure after large-scale breeding and processing industry This not only causes serious environmental pollution, but also causes a large amount of nutrient resources (C, N, P, K, S and trace elements) to be lost. Since the mid-1990s, the disposal of agricultural organic waste has become a severe problem facing the vast rural ecological environment in our country. The resourceful treatment of agricultural waste is of great significance to alleviate the shortage of resources...

AI Technical Summary

Problems solved by technology

A large number of studies at home and abroad have shown that the isolation and screening of microorganisms with strong degradation ability has a good application prospect for solving the utilization of agricultural waste resources, and the current research mainly focuses on the research on the degradation of a single strain, but the enzyme production of a single strain Small, low degradation rate, difficult to popularize

Moreover, agricultural waste straw is mainly composed of macromolecular substances such as cellulose, protein, and sugar. Many scholars focus on the research on the degradation of a single species, which often makes the degradation of straw incomplete.

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