Bacillus subtilis strain usable as probiotic preparation and fermentation process thereof

Abstract

The invention discloses a Bacillus subtilis strain usable as a probiotic preparation and a fermentation process thereof. The fermentation process comprises the following steps: step 1, inoculating an inclined medium with the Bacillus subtilis strain and carrying out culture for 3 days in a thermotank with a temperature of 37 DEG C, wherein the obtained substance is used as a seed culture; and step 2, inoculating a glucose nutritional broth with the seed culture, carrying out culture at 37 DEG C for 24 h, mixing the obtained solution with light calcium carbonate according to a ratio of 1: 3, drying the obtained mixture and then crushing the mixture. Compared with the prior art, the invention has the following beneficial effects: the Bacillus subtilis strain BB has high enzyme production capability, is applicable as a feed additive to feeds for livestock and poultry, can improve production performance of bred animals and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus subtilis which can be used as a probiotic preparation and a fermentation process thereof. Background technique [0002] In livestock and poultry breeding, antibiotics are widely used in livestock and poultry feed because they can effectively reduce the incidence of livestock and poultry and improve the growth efficiency of livestock and poultry. However, the problems caused by the abuse of antibiotics have attracted increasing attention, such as the increase of drug-resistant pathogens, excessive drug residues in livestock and poultry products, affecting the micro-ecological balance in the intestinal tract of livestock and poultry, and the safety of livestock and poultry food. In this regard, the EU has completely banned the use of antibiotic growth-promoting feed additives in feed. In order to deal with these problems, the use of probiotic microecological pr...

AI Technical Summary

Problems solved by technology

However, the problems caused by the abuse of antibiotics have attracted increasing attention, such as the increase of drug-resistant pathogens, excessive drug residues in livestock and poultry products, affecting the micro-ecological balance in the intestinal tract of livestock and poultry and the safety of livestock and poultry food, etc.