Method for cooperatively treating formaldehyde by microorganism and enzyme

A collaborative treatment and microbial technology, applied in the direction of microbial-based methods, biochemical equipment and methods, biological water/sewage treatment, etc., can solve the problems of low efficiency, achieve the effect of improving degradation rate and good industrial application value

Inactive Publication Date: 2016-02-10
广州荣天环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for synergistic treatment of formaldehyde by microorganisms and enzymes in order to overcome the problem of low efficiency of the existing formaldehyde-degrading microorganisms in the process of removing formaldehyde

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] S1. Prepare liquid medium; mix peptone 10g / L, yeast extract 5g / L and NaCl10g / L, adjust the pH to 7.0, and sterilize at high temperature;

[0027] S2. Inoculation of formaldehyde-degrading microorganisms: the formaldehyde-degrading microorganisms are cultured on a slant to make a spore suspension with a spore concentration of 109 / ml, then inoculate 5% by weight of the spore suspension into a liquid medium containing 500mg / ml formaldehyde, and then add 0.5% by weight of catalase;

[0028] S3. Degradation of formaldehyde: Under the condition of 30° C., shake and cultivate for 24 hours on a shaker with a rotation speed of 150 r / min. Determination of formaldehyde content.

Embodiment 2

[0034] S1. Prepare liquid medium; mix peptone 10g / L, yeast extract 5g / L and NaCl10g / L, adjust the pH to 7.0, and sterilize at high temperature;

[0035] S2. Inoculation of formaldehyde-degrading microorganisms: culture the formaldehyde-degrading microorganisms on a slant to make a spore suspension with a spore concentration of 10 9 / ml, then inoculate 5% by weight of the spore suspension into the liquid medium containing 1000mg / ml formaldehyde, then add 0.5% by weight of catalase;

[0036] S3. Degradation of formaldehyde: Under the condition of 30° C., shake and cultivate for 24 hours on a shaker with a rotation speed of 150 r / min. Determination of formaldehyde content.

Embodiment 3

[0042] S1. Prepare liquid medium; mix peptone 10g / L, yeast extract 5g / L and NaCl10g / L, adjust the pH to 7.0, and sterilize at high temperature;

[0043] S2. Inoculation of formaldehyde-degrading microorganisms: culture the formaldehyde-degrading microorganisms on a slant to make a spore suspension with a spore concentration of 10 9 / ml, then inoculate 5% by weight of the spore suspension into the liquid medium containing 3000mg / ml formaldehyde, then add 0.5% by weight of catalase;

[0044] S3. Degradation of formaldehyde: Under the condition of 30° C., shake and cultivate for 24 hours on a shaker with a rotation speed of 150 r / min. Determination of formaldehyde content.

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PUM

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Abstract

The invention relates to the technical field of environmental friendless and particularly discloses a method for cooperatively treating formaldehyde by a microorganism and an enzyme. The method comprises the following steps: S1, preparing a liquid culture medium; S2, inoculating a formaldehyde degrading microorganism: carrying out inclined plane culture on the formaldehyde degrading microorganism to prepare a spore suspended solution, wherein the concentration of spores is 0.1*10<9> / ml-10*10<9> / ml; then inoculating the spore suspended solution into the liquid culture medium containing the formaldehyde, and adding catalase; and S3, degrading the formaldehyde: under the conditions of 25-35 DEG C, carrying out vibration culture for 18-36 hours. According to the method provided by the invention, the cooperative effect is generated by the catalase and the formaldehyde degrading microorganism, so that the degradation rate of the formaldehyde is improved and the method has a very good industrial application value.

Description

technical field [0001] The invention relates to the technical field of environmental protection, in particular to a method for synergistically treating formaldehyde with microorganisms and enzymes. Background technique [0002] Formaldehyde is a colorless organic pollutant. Its main sources of pollution come from exhaust gases such as organic synthesis, paints, decoration materials, and paints. Formaldehyde is prone to polyaddition and redox reactions. It can cause damage to the system, immune system, liver, etc. when inhaled by the human body. All are poisonous and are Class I carcinogens, so it is very necessary to prevent and control pollutants such as formaldehyde in household and decoration. [0003] At present, the methods for removing formaldehyde pollution include activated carbon adsorption, nano-photocatalysis, ozone negative ions, plant decomposition, plasma technology, etc., but these methods consume a lot of chemicals, are expensive, and have low removal rates. ...

Claims

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Application Information

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IPC IPC(8): B01D53/84B01D53/72C02F3/34A62D3/02C12N1/20C12N1/14C12R1/38C12R1/07C12R1/66C12R1/40C12R1/125C12R1/685C12R1/67
CPCY02A50/20
Inventor 孙婷付鹏
Owner 广州荣天环保科技有限公司
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