Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine

A time-resolved fluorescence and amantadine technology, which is applied in the analysis of materials, fluorescence/phosphorescence, and material excitation analysis, can solve the problems of low sensitivity, affect the implementation of national animal disease prevention and control policies, and small stock displacement, etc., and achieve high sensitivity Effect

Inactive Publication Date: 2016-02-03
JIANGSU WISE SCI & TECH DEV
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Problems solved by technology

[0006] In 2005, the Chinese government's "Announcement of the Ministry of Agriculture (No. 560)" also clearly pointed out that "amantadines and other antiviral drugs for human transplantation lack scientific norms and safe and effective experimental data. Animal disease control brings adverse consequences and affects the implementation of national animal disease prevention and control policies
The rapid detection products developed based on colloidal gold labeling technology have problems such as qualitative or semi-quantitative, and large batch-to-batch differences; although the batch-to-batch difference of colored latex microspheres has improved, the sensitivity is still low and can only be qualitative or semi-quantitative ; The sensitivity of immunochromatography based on fluorescein labeling technology has been greatly improved, and quantitative detection can also be performed, but because the sample contains a high fluorescent background signal and the stock shift is small, it will have a greater impact on the detection

Method used

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  • Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine
  • Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine
  • Preparation method and application of test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine

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Experimental program
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Embodiment Construction

[0040] 1. Preparation of each component of the test strip

[0041] 1.1 Preparation of quality control microspheres Preparation of biotin-labeled γ-globulin (BGG)-coated quality control microspheres

[0042] (1) Preparation of biotin-labeled BGG

[0043] with 0.1M NaCNBH 3 Prepare BGG (purchased from Pel-Freez Biological) into a 10 mg / mL solution, and use DMSO (dimethylformamide) to configure Biotin-X-X-NHS (N-hydroxysuccinimide modified biotin, manufacturer: SIGMA, product number : B3295) solution to 16.2mg / mL, add Biotin-X-X-NHS solution to the BGG solution according to the amount of 5.4μ1 Biotin-X-X-NHS added to 1mgBBG protein, mix well and place overnight at 4°C. Dialysis was used to remove free unreacted biotin, and the dialysate was biotin-labeled protein dialysate buffer (0.1MTris, 0.3MNacl, 0.005MEDTA-Na-2H 2 0, pH8.0). After dialysis, the protein concentration was determined by BCA method.

[0044] ⑵Using the above-mentioned labeled protein to coat the aldehyde-modi...

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Abstract

The invention relates to a preparation method and application of a test strip for fast time-resolved fluorescent immunochromatography quantitative detection of amantadine. The test strip comprises three parts such as a Fusion 5 membrane, a cellulose nitrate membrane and water absorbent paper. According to the principle of the competition law, a time-resolved fluorescent microsphere is taken as an immune marker for accurate and fast quantitative detection of an antigen to be detected.

Description

technical field [0001] The invention belongs to the field of food safety detection, and in particular relates to a detection method for harmful residues in food, in particular to a time-resolved fluorescent immunochromatographic test strip for quantitative detection of amantadine, a preparation method and application thereof. Background technique [0002] Amantadine is the earliest antiviral drug used to inhibit influenza virus, U.S. It was approved as a preventive medicine in 1966 when the Asian flu was prevalent. And in 1976, it was confirmed as a therapeutic drug on the basis of a preventive drug. The efficacy and safety of the drug on adult patients have been widely recognized. However, the therapeutic dose is very close to the dose that produces side effects, and it is difficult to determine the dose and administration plan for the elderly and those with chronic cardiopulmonary disease or kidney disease, so it has not been widely used in clinical practice. exist Ja...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N21/64
Inventor 杜道林邢海龙洪霞
Owner JIANGSU WISE SCI & TECH DEV
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