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Preparation method for high-specificity difenoconazole polyclonal antibody

A technology of difenoconazole and polyclonal antibodies, which is applied to the preparation method of peptides, chemical instruments and methods, specific peptides, etc., and can solve the problems of expensive equipment, complicated pretreatment, and inability to detect simultaneously

Active Publication Date: 2016-02-03
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the accumulation of toxicity is not strong, if the residual amount is higher than the residual limit standard, it will also cause serious harm to the human body
[0004] At present, the commonly used detection methods of difenoconazole include gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS). Complicated, expensive instruments and corresponding supporting equipment, unable to perform simultaneous detection of parallel samples on a large scale, etc.
Enzyme-linked immunoassay has the advantages of simple method, convenient speed, strong specificity, etc. It has not been widely used in the detection of difenoconazole residues, and has a good development prospect

Method used

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  • Preparation method for high-specificity difenoconazole polyclonal antibody
  • Preparation method for high-specificity difenoconazole polyclonal antibody
  • Preparation method for high-specificity difenoconazole polyclonal antibody

Examples

Experimental program
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Embodiment 1

[0041] Synthesis of Difenoconazole Hapten

[0042] Weigh 6.99g [BMIM]Cl into a dry 100mL three-neck flask, slowly add 12.975g of anhydrous ferric chloride solid to it, and react overnight under nitrogen protection to obtain [BMIM]Cl-FeCl 3 Ionic liquid: add 10mL 3,4'-dichlorodiphenyl ether and 7mL bromoacetyl chloride directly into the reacted ionic liquid, install a condenser tube, install a calcium chloride water absorption device on the top of the condenser tube, and store at 60°C Heating to reflux for 5 hours, after the reaction, transfer the reaction system to a beaker, extract the product with cyclohexane, and spin the extract to dryness at 45°C; use petroleum ether and ethyl acetate as developing solvents to pass the above product through a silica gel column Separate and purify to obtain the intermediate 2-bromo-1-(2-chloro-4-(4-chlorophenoxy)phenyl)ethanone; respectively weigh 9.73mg 4-aminobutyric acid, 5mg sodium bicarbonate, and 10mg potassium iodide , put into a 5...

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Abstract

The invention provides a preparation method for a high-specificity difenoconazole polyclonal antibody, and belongs to the immune analysis technology. The polyclonal antibody is prepared by the steps of combining a difenoconazole hapten with a carrier protein and immunizing animals, wherein the difenoconazole hapten is prepared by carrying out a reaction of 3,4'-dichlorodiphenyl ether and bromoacetyl chloride to obtain an intermediate, and then connecting with 4-aminobutyric acid. The prepared difenoconazole polyclonal antibody has higher specificity, and fills up a blank in a research direction of preparation of the high-specificity difenoconazole polyclonal antibody.

Description

technical field [0001] The invention belongs to the technical field of immune analysis of small molecule compounds, and relates to a preparation method of a highly specific polyclonal antibody of triazole fungicides. Background technique [0002] Difenoconazole, also known as difenoconazole, is a broad-spectrum and high-efficiency triazole systemic fungicide, which can strongly inhibit the biosynthesis of ergosterol, an important component of the cell membrane of pathogenic bacteria, thereby destroying the biosynthesis of the cell membrane. Structure and function, long-lasting protective and therapeutic effects on crops. [0003] Difenoconazole is a low-toxicity fungicide, and the acceptable daily intake for humans is 0.01mg / kg body weight. According to the study on the acute toxicity of difenoconazole, the acute oral median lethal dose (LD50) of this drug in rats is 1453 mg / kg, and the acute percutaneous LD50 in rabbits is greater than 2010 mg / kg. It is irritating to the ...

Claims

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Application Information

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IPC IPC(8): C07K16/44C07K1/34G01N33/53
Inventor 刘冰冯久慧王硕
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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