A method for rapidly obtaining excellent hybrid clones of iris

Abstract

The invention discloses a method for rapid acquisition of an excellent hybrid clone of iris, belonging to the fields of plant tissue culture and plant biotechnology. According to the method, a mature hybrid seed of iris is used as an object and undergoes induced sprouting and subculturing propagation in virtue of embryo culture technology so as to obtain a great number of hybrid rootless sprouts; a part of the hybrid rootless sprouts are subjected to subculturing so as to retain germplasm; the other part of the hybrid rootless sprouts are subjected to strong sprout rooting, acclimatization and transplant and field planting, and after a growth period, the excellent hybrid clone achieving breeding objectives is screened; and the screened excellent hybrid clone is rapidly propagated to a certain scale through recovery growth in virtue of the retained germplasm. Compared with a conventional method which sows seeds at first, then screens out excellent individual plants and finally carries out tissue culture and propagation, the method provided by the invention enables a great number of seedlings of the excellent hybrid clone to be obtained 2 years earlier, so breeding process is substantially shortened.

Description

technical field [0001] The invention belongs to the field of plant tissue culture and plant biotechnology, and in particular relates to a method for rapidly obtaining excellent hybrid clones of iris. Background technique [0002] Iris ( Iris ), Iridaceae (Iridaceae) perennial flowers, species and varieties. The flower color is rich, the flower shape is different, the leaves are green and green, and the ornamental value is very strong. Some varieties have light and elegant flowers, and their rhizomes can be used to extract essence and be used as traditional Chinese medicine. Multi-use division, sowing method propagation. [0003] The breeding research of Iris plants in my country began in the 1990s. The seed setting rate of hybridization between iris varieties is low, and the seeds generally have dormancy, the dormancy period is long, the emergence rate is low, and the ramet reproduction coefficient is low, and the time required for seedling propagation is long; the expla...

AI Technical Summary

Problems solved by technology

The seed setting rate of hybridization between iris varieties is low, and the seeds generally have dormancy, the dormancy period is long, the emergence rate is low, and the ramet reproduction coefficient is low, and the time required for seedling propagation is long; the explants of iris tissue culture mostly use flower buds, but It takes 2-3 years for seedlings to flower, and with few basic materials, it is difficult to obtain a large number of explants in a short period of time, which greatly limits the progress of tissue culture and propagation of iris

Therefore, the process of iris breeding has been greatly affected