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Method of nutritional enhancement of rotifers with Antarctic krill meal

An Antarctic krill and nutritional enhancement technology, applied in the application, animal feed, animal feed and other directions, to achieve the effect of not easily polluting water quality, simple operation and easy to use

Inactive Publication Date: 2014-08-27
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Antarctic krill powder is rich in nutrients such as protein, amino acid, fatty acid, vitamins, etc. However, there is no report about nutrition enrichment of rotifers in Antarctic krill powder

Method used

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  • Method of nutritional enhancement of rotifers with Antarctic krill meal

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Embodiment Construction

[0008] The present invention uses Antarctic krill powder as the nutrient material for strengthening rotifers, and the contents of DHA, EPA, ARA and ω3-PUFA in the fatty acid composition in Antarctic krill powder are (16.86±2.12)%, (23.54 ±2.36)%, (0.17±0.02)% and (42.56±4.25)%;

[0009] The preparation step of mother liquor of Antarctic krill meal is to first use a cross-stirring grinder to ultrafinely pulverize the Antarctic krill meal. Finely pulverize the Antarctic krill powder so that the particle size is less than 120μm, then add 100g of the ultrafinely pulverized Antarctic krill powder to 600mL of pure water and stir with a homogenizer. The speed of the homogenizer is 15500- 22000rpm, stirring time is 2min, then repeated stirring 3 times according to the same method, and then ultrasonic crushing and dissolving for 20min, so that the particle size of Antarctic krill powder is less than 25μm, and the mother liquor of Antarctic krill powder is obtained, and the Antarctic kr...

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Abstract

The invention relates to a method of nutritional enhancement of rotifers with Antarctic krill meal. The Antarctic krill meal is used as nutrient material for enhancement of the rotifers. The method is characterized in that the Antarctic krill meal is ground in a superfine mode with a cross-stirring grinder to enable the grain size to be smaller than 120 millimeters, 100g of the Antarctic krill meal after the superfine grinding is added into 600mL of purified water in a proportional mode, the solution is stirred through a homogenizer, the Antarctic krill meal is further crushed and dissolved by supersonic waves to enable the grain size of the Antarctic krill meal to be smaller than 25 millimeters, an Antarctic krill meal mother solution is obtained, the total volume of the mother solution is replenished to 1L with purified water, and then the mother solution is stored at 4 DEG C in a refrigerator. The steps of enhancement of the rotifers are that the rotifers are collected into a barrel containing clean sea water, the water temperature is (25+ / -0.5) DEG C, the salinity is 28+ / -1, aeration is carried out continuously, natural lighting is imposed, the density of the rotifers is (550+ / -10) inds / mL, the Antarctic krill meal mother solution obtained is added to the barrel, the final concentration of the Antarctic krill meal is 50mg / L, and the enhancement time is 6-18h.

Description

technical field [0001] The invention belongs to the nutritional enhancement technology of aquatic organism bait, in particular to a method for nutritionally strengthening rotifers with Antarctic krill powder. Background technique [0002] Brachionus plicatilis (hereinafter referred to as rotifer) is a small multicellular aquatic animal widely distributed in brackish water and seawater in temperate tropical regions, and is an important natural source of fish and crustaceans. Biological bait has been widely used in the cultivation of seawater larvae, the production of shrimp and crab seedlings, and the cultivation of precious ornamental aquarium animals. At present, in the large-scale productive cultivation of rotifers, yeast is mainly used as a suitable bait, and the rotifers cultivated with yeast as bait lack ω3-series polyunsaturated fatty acids (ω3-PUFA), especially 22 Carbasahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). The research results of aquatic animal nut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033A23K1/18A23K1/10
Inventor 陆建学林听听高露姣黄艳青
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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