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Detection method for compound radix salviae miltiorrhizae dripping pills through quantitative analysis of multiple components by single marker

A technology of compound salvia miltiorrhiza and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2016-01-27
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the shortcomings of the existing single-component and multi-indicator content determination methods, the present invention provides a highly practical, easy-to-operate and cost-saving detection method for the six phenolic acid components of Compound Danshen Dripping Pills

Method used

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  • Detection method for compound radix salviae miltiorrhizae dripping pills through quantitative analysis of multiple components by single marker
  • Detection method for compound radix salviae miltiorrhizae dripping pills through quantitative analysis of multiple components by single marker
  • Detection method for compound radix salviae miltiorrhizae dripping pills through quantitative analysis of multiple components by single marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0274] 1) Compound Danshen dripping pills are prepared into test solution;

[0275] Take 25 capsules of Compound Danshen Dripping Pills, weigh them accurately, add 20mL of di-pure water to a 25mL measuring bottle, ultrasonically (300W, 50kHz) for 10min, wait to cool to room temperature, dilute to 25mL, pass through a 0.22μm filter membrane, and wait for Test samples.

[0276] 2) Determining the protocatechualdehyde content in the test solution with an ultra-high performance liquid chromatograph;

[0277] Take an appropriate amount of protocatechuic aldehyde reference substance, weigh it accurately, and prepare a reference solution with a concentration of about 40 μg / mL

[0278] Sample determination, using UPLC to determine the peak area of ​​the analyte in the sample solution and the reference solution,

[0279] Chromatographic conditions

[0280] Chromatographic column: ACQUITYUPLCBEHC18 (1.7μm, 2.1mmi.d.×100mm), WatersACQUITYUPLCBEHC18 pre-column (1.7μmparticles, 2.1mmi.d...

Embodiment 2

[0297] 1) Compound Danshen dripping pills are prepared into test solution;

[0298] Take 25 capsules of Compound Danshen Dripping Pills, weigh them accurately, add 20mL of di-pure water to a 25mL measuring bottle, ultrasonically (300W, 50kHz) for 10min, wait to cool to room temperature, dilute to 25mL, pass through a 0.22μm filter membrane, and wait for Test samples.

[0299] 2) Determining the protocatechualdehyde content in the test solution with an ultra-high performance liquid chromatograph;

[0300] Use ultra-high performance liquid chromatography to measure the peak areas of protocatechualdehyde in the sample solution to be tested and the reference substance solution, and calculate the content of protocatechualdehyde by the working curve method; five gradients of protocatechualdehyde in the concentration range of 10-100 μg / mL are used. Catechin aldehyde was used as the reference solution.

[0301] Column:; or DikmaEndeavorsil column

[0302] Mobile phase selection:

...

Embodiment 3

[0317] 1) Compound Danshen dripping pills are prepared into test solution;

[0318] Compound Danshen dripping pills were prepared into a test solution; the method was as follows: Take 30 compound Danshen dripping pills, weigh them accurately, add 25mL water into a 30mL measuring bottle, and ultrasonically 15min, wait to cool to room temperature, dilute to 30mL, pass through 0.3 μm filter membrane, the filtrate obtained is the liquid to be tested

[0319] 2) Determining the protocatechualdehyde content in the test solution with an ultra-high performance liquid chromatograph;

[0320] Use ultra-high performance liquid chromatography to measure the peak area of ​​protocatechualdehyde in the sample solution to be tested and the reference substance solution respectively, and use the external standard one-point method, wherein the external standard one-point method uses protocatechualdehyde with a concentration of 40 μg / mL as the reference substance ,

[0321] Chromatographic colu...

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Abstract

The invention relates to a detection method for compound radix salviae miltiorrhizae dripping pills through quantitative analysis of multiple components by single marker; the method is a content determination method for six kinds of phenolic acid components through UPLC and quantitative analysis of multiple components by single marker; protocatechualdehyde is adopted as a single calibration object in experiments, and determination is carried out through a method with an external standard method and a correction factor; the method provides an effective and accurate quality control method for pharmacodynamic substances of the compound radix salviae miltiorrhizae dripping pills.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a one-test, multiple-evaluation detection method for compound danshen dripping pills. The method includes a UPLC one-test, multiple-evaluation content determination method for six phenolic acid components. Background technique [0002] Due to the complexity of phytochemical components, multi-index content determination has become a consensus for the quality control of plant-derived drugs. At present, more and more domestic and foreign botanical drug standards include multi-index content determination. However, in the process of implementing the multi-index content determination method, the consumption of reference substances is large, and the detection operation is difficult, which seriously restricts the popularization and application of this method. [0003] To solve the above problems, people are actively looking for alternative methods. The researchers found that the ratio of the ...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 佟玲周水平孙鹤孙万阳黄竞怡
Owner TIANJIN TASLY PHARMA CO LTD
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