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Method of preparing microbial oil and microbial oil

A technology for microbial oil and oil-producing microorganisms, which is applied in the production of fat, fat oil/fat production, fermentation, etc., to achieve the effect of reducing surface oil content, promoting emulsification process, and reducing preparation process

Inactive Publication Date: 2016-01-27
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing enzymatic degumming process is carried out after the crude oil is obtained

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] With dinoflagellation as the fermentation strain, follow the following steps successively:

[0017] (1) fermentation to obtain a microbial fermentation broth rich in docosahexaenoic acid;

[0018] (2) After the above-mentioned fermented liquid is processed by a centrifuge, the concentrated fermented liquid obtained with a solids content of 25.1%;

[0019] (3) Take 500g of the concentrated fermentation broth and put it into a 1L glass reaction bottle, adjust the pH value to 8.01, add 1.01g of alkaline protease, control the temperature at 45°C, cut for 2h, then add 2.5g of phospholipase C, and stir further React for 2 hours to obtain the enzymolysis solution.

[0020] (4) The above enzymolysis solution was heated to 97° C., and then centrifuged with a high-speed centrifuge at 10,000 rpm to separate the upper oil phase for vacuum dehydration to obtain 41.3 g of microbial oil rich in docosahexaenoic acid.

[0021] (5) After testing, the phosphorus content in the microbial...

Embodiment 2

[0023] Using Microella as the fermentation strain, follow the steps below:

[0024] (1) fermentation to obtain a microbial fermentation broth rich in eicosapentaenoic acid;

[0025] (2) After the above-mentioned fermented liquid is separated by membrane, the concentrated fermented liquid obtained with a solids content of 17.1%;

[0026] (3) Take 5kg of the concentrated fermentation broth and put it into a 25L glass reactor, adjust the pH value to 7.01, add 5.1g alkaline protease, 5.1g helicase, control the temperature at 51°C, 10g phospholipase C, stir and shear React for 4 hours to obtain the enzymolysis solution.

[0027] (4) 10 L of n-hexane and 5 L of ethanol were added to the enzymolysis solution for extraction for 1 hour, and the extract was separated by sedimentation to obtain an extract, which was subjected to vacuum precipitation to obtain 377.3 g of microbial oil rich in eicosapentaenoic acid.

[0028] (5) After testing, the phosphorus content in the microbial oil ...

Embodiment 3

[0030] Using yeast as the fermentation strain, follow the steps below:

[0031] (1) Fermentation obtains a microbial fermentation broth rich in linolenic acid;

[0032] (2) After the above-mentioned fermented liquid is centrifuged, the concentrated fermented liquid obtained with a solids content of 29.7%;

[0033] (3) Take 51kg of the concentrated fermentation broth and put it into a 300L reactor, adjust the pH value to 5, control the temperature at 48°C, add 100.5g of helicase and 127.5g of phospholipase C, stir and cut for 2 hours to obtain an enzymolysis solution.

[0034] (4) Add 100L of n-hexane and 50L of ethanol to the above enzymatic hydrolysis solution for extraction for 1.5h, settle and separate to obtain the extract, and vacuum precipitate the extract to obtain 4179.1g of microbial oil rich in linolenic acid.

[0035] (5) After testing, the phosphorus content in the microbial oil is 2.9ppm, the triglyceride content is 94.1%, and the diglyceride content is 3.9%.

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Abstract

A method of preparing microbial oil includes the steps: performing fermenting to obtain an oil-producing microbial fermentation liquor; concentrating the fermentation liquor to obtain concentrated fermentation liquor having a solid content higher than 10%; adjusting pH value of the concentrated fermentation liquor to 5-8.1, adding phospholipase C into the fermentation liquor, controlling the temperature to be 38 DEG C to 51 DEG C, and performing stirring and cutting for 2-6 h; separating an oil phase and an aqueous phase to obtain the microbial oil having a phosphorus content lower than 5ppm. The degumming procedure and the microbial wall-breaking procedure are combined, so that preparation procedures of the oil are decreased and production cost is lowered; further, the degumming procedure is set before the preparation procedure of crude oil, so that the produced crude oil has higher quality. In another aspect, the phospholipase C can hydrolyze glycerol phospholipids into diglyceride, so that the content of the diglyceride in the oil is increased; since the diglyceride has better emulsifying performance, the emulsifying process for preparing microbial oil microcapsules is facilitated, the embedding rate of the microbial oil can also be increased, thus lowering the surface oil content of the microcapsules and improving antioxidation of the microcapsules.

Description

technical field [0001] The invention relates to a method for preparing microbial oil and microbial oil prepared by the preparation method. Background technique [0002] At present, microbial oil is obtained by fermenting microbial strains to obtain a large number of microorganisms, and then performing pretreatment, and obtaining microbial crude oil through organic solvent extraction and precipitation. The microbial crude oil already contains specific microbial oil components, so it can be sold as a product on the market. However, the microbial crude oil has many impurities and is not good in sense. Therefore, it is often necessary to further refine it to obtain microbial oil with better quality. Oil refining is usually divided into degumming, alkali refining, decolorization, deodorization and other processes. Among them, degumming is the basis of the entire oil refining process, and its main function is to remove phospholipids (hydratable phospholipids and non-hydratable ph...

Claims

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Application Information

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IPC IPC(8): C12P7/64C11B1/00
Inventor 汪志明陆姝欢李翔宇田勇周强易德伟
Owner CABIO BIOTECH WUHAN CO LTD
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