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Plum SSR labeled primer pair exploited on basis of transcriptome sequence, and application thereof

A technology of transcriptome sequence and labeled primers, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, biochemical equipment and methods, etc., to achieve the effect of increasing raw data and improving the efficiency of development

Active Publication Date: 2016-01-13
POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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Problems solved by technology

[0004] The purpose of the present invention is to provide the Lee SSR marker primer and its application based on the development of the transcriptome sequence for the lack of the current lack of Lee SSR marker primers

Method used

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  • Plum SSR labeled primer pair exploited on basis of transcriptome sequence, and application thereof
  • Plum SSR labeled primer pair exploited on basis of transcriptome sequence, and application thereof
  • Plum SSR labeled primer pair exploited on basis of transcriptome sequence, and application thereof

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Embodiment 1

[0058] The Li SSR primer pair developed based on transcriptome sequencing was obtained through the processes of Li transcriptome sequencing, SSR site identification, SSR primer design, DNA extraction, SSR primer screening, and genetic diversity analysis.

[0059] In the present invention, the specific method of the Li SSR primer pair developed based on the transcriptome sequence is:

[0060] 1) Acquisition of transcriptome data

[0061] The total RNA of Prunus hibiscus fruit was extracted by EZNAPlantRNAKit (OmegaBio-tek). Qubit2.0Fluorometer (Invitrogen, LifeTechnologies, CA, USA) was used to measure the concentration of RNA, and Agilent2100Bioanalyzer (AgilentTechnologies, Santa Clara, CA, USA) was used to evaluate the quality of RNA. RNAs from fruit samples of the same stage from three different trees were mixed in equal amounts and used for sequencing library construction.

[0062] The total RNA of Hibiscus plum fruit was extracted by EZNAPlantRNAKit (OmegaBio-tek), and ...

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Abstract

The invention belongs to the fields of molecular marker technology exploitation and application, and concretely provides a plum SSR labeled primer pair exploited on the basis of a transcriptome sequence, and an application thereof in plum germplasm resource researches. The primer pair is exploited on the basis of the transcriptome sequence, batch treatment of a large amount of sequence information is carried out on the basis of transcriptome sequencing, and large batch exploitation of SSR labels can be realized through SSR site searching and SSR primer designing, so the exploitation efficiency is greatly improved, and the problems of complex steps, large workload and high exploitation cost of traditional SSR label exploitation methods are solved. The validity of SSR primers is verified through SSR primer screening and diversity analysis. Foundation is laid for plum SSR labeling primer exploitation and researches of the plum germplasm resource diversity, the kind identification and the genetic relationship by using the SSR molecular labels.

Description

technical field [0001] The invention belongs to the field of development and application of molecular marker technology, and in particular relates to a pair of SSR marker primers developed based on transcriptome sequences and a method for developing SSR primers. Background technique [0002] Prunus is a plant of the genus Prunus in the family Rosaceae, which is widely distributed in countries all over the world and is also one of the important traditional fruit trees in my country. Plum fruit is rich in biologically active substances such as carotenoids, vitamins and anthocyanins, which have the effect of preventing various diseases and are deeply loved by consumers. my country's plum germplasm resources are extremely rich, there are many varieties of plums, unscientific naming, and the phenomenon of the same name and different species or the same thing with different names is very serious, which seriously hinders the development and utilization of plum germplasm resources. ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 方智振叶新福周丹蓉姜翠翠潘少霖
Owner POMOLOGY RES INST FUJIAN ACAD OF AGRI SCI
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