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An in vitro culture system kit for improving human sperm motility and its application

An in vitro culture system and in vitro culture technology, applied in the field of in vitro culture system kits, can solve the problems of ineffective improvement of sperm motility, achieve the effects of improving forward motility, increasing fertilization rate, and improving pregnancy outcomes

Active Publication Date: 2018-11-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned culture medium mainly provides a buffer and a basic energy supply environment for sperm, and has no obvious effect on improving sperm motility

Method used

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  • An in vitro culture system kit for improving human sperm motility and its application
  • An in vitro culture system kit for improving human sperm motility and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Sample source: three normal motility sperm samples (the standard is according to the fifth edition of WHO)

[0024] Sperm processing:

[0025] 1. Preheat 10 mL of mHTF containing 10% SSS by volume fraction (as a control group before cultivation), 2 mL of Isolate sperm separation liquid and 10 mL of in vitro culture liquid of the present invention.

[0026] 2. Place the semen collection cup in a 37°C water bath, and take it out after liquefaction.

[0027] 3. Mix the preheated mHTF with a volume fraction of 10% SSS and the liquefied semen, slowly add it to the Isolate sperm separation solution, and centrifuge at 300g for 10 minutes.

[0028] 4. Discard the supernatant after centrifugation, remove the lower layer and separate the sperm for microscopic examination, record the sperm motility, and serve as the control group before culture.

[0029] 5. Mix the remaining sperm in step 4 with the sperm in vitro culture solution of the present invention at a concentration of 1...

Embodiment 2

[0033] Sample source: three sperm samples from patients with asthenozoospermia (the standard is according to the fifth edition of WHO)

[0034] Sperm processing:

[0035] 1. Preheat 10 mL of mHTF containing 10% SSS by volume fraction (as a control group before cultivation), 2 mL of Isolate sperm separation liquid and 10 mL of in vitro culture liquid of the present invention.

[0036] 2. Place the semen collection cup in a 37°C water bath, and take it out after liquefaction.

[0037] 3. Mix the preheated mHTF with a volume fraction of 10% SSS and the liquefied semen, slowly add it to the Isolate separation solution, and centrifuge at 300g for 10 minutes.

[0038] 4. Discard the supernatant after centrifugation, remove the lower layer and separate the sperm for microscopic examination, record the sperm motility, and serve as the control group before culture.

[0039] 5. Mix the remaining sperm in step 4 with the sperm culture system of the present invention at a concentration ...

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Abstract

The invention discloses an in vitro culture system kit for raising human sperm motility and an application thereof and belongs to the field of XX technology. The kit comprises 9mL of mHTF, 25 microliters of Hexadecanoic Acid, 25 microliters of Trans-13-Octadecenoic Acid, 20 microliters of 9,12-Octadecenoic, 30 microliters of Arachidonic Acid and 50 microliters of Docosahexaenoate Acid. The reagents in the kit are mixed and balanced at 37 DEG C for 2-3 h so as to obtain 10.15 ml of a sperm culture system; and sperm with the concentration of 10 millon / mL that is separated by a sperm split liquid is resuspended by the use of the above sperm culture system, blending is lightly carried out, and the product is put in a thermotank of 37 DEG C for 90-120 min, so as to achieve the effect of raising sperm motility. By the use of the kit, sperm progressive motility can be remarkably raised. When the kit is used in the technologies of artificial insemination and in vitro fertilization, pregnancy outcome can be improved, and rate of fertilization can be raised. Meanwhile, a new method is provided for treatment of patients with severe asthenospermia.

Description

technical field [0001] The invention relates to an in vitro culture system kit for improving human sperm motility and its application, belonging to the technical field of biomedicine. Background technique [0002] According to the data of the World Health Organization, about 15% of couples of childbearing age in the world are facing the threat of infertility. The recent survey results in my country show that domestic infertility accounts for 10% of the number of married couples, and the incidence rate is on the rise. Among them, about half of human infertility patients are male infertility patients, and asthenospermia is one of the common causes of male infertility. Clinically, artificial insemination is often used for treatment. Data show that better sperm motility is an important condition for improving the success rate of assisted reproductive technology. [0003] The main components of semen are sperm and seminal plasma. Sperm originate from the testes and are the main ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/076
Inventor 王英男杨勇吴亦波毛健
Owner JIANGNAN UNIV
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