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Lincomycin hydrochloride extraction method

A technology of lincomycin hydrochloride and extraction method, which is applied in the field of extracting lincomycin hydrochloride by using aqueous two-phase extraction technology

Active Publication Date: 2016-01-13
NINGXIA TAIYICIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no process technology for extracting lincomycin hydrochloride using two-phase aqueous phase that has been publicly reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take 300L of lincomycin fermentation broth, the potency of the fermentation broth is 7420u / ml, add 5.5kg of oxalic acid under stirring, adjust the pH to 2.5~3.5, and add 0.36kg of yellow blood salt and 0.36kg of zinc sulfate in sequence at intervals of 20 minutes, and continue Stir for 30 minutes, filter with plate and frame, and wash with oxalic acid or PH2.5-3.5 aqueous solution prepared with 2mol / L HCL to obtain 330L of filtrate, with a potency of 6560u / ml and a yield of 97.25%.

[0034] According to the volume ratio of 16%PEG2000 and filtrate washing solution 1:3, take 110L prepared 18%K 2 HPO 4 and 16% PEG2000 solution, stir and mix, let it stand for 10 minutes to form a two-phase system, add the above filtrate to the two-phase system under stirring, adjust the pH to 9.5-10.0 with 10% liquid alkali, and stir for 15 minutes , let stand for 40 minutes, layered, the volume of the upper layer is 108L, and the upper layer and the lower layer are respectively sampled to...

Embodiment 2

[0040] Take 300L of lincomycin fermentation broth, the potency of the fermentation broth is 7560u / ml, add 5.7kg of oxalic acid under stirring, adjust the pH to 2.5~3.5, and add 0.36kg of yellow blood salt and 0.36kg of zinc sulfate in sequence at intervals of 20 minutes, continue Stir for 30 minutes, filter with plate and frame, and wash with oxalic acid or PH2.5-3.5 aqueous solution prepared with 2mol / L HCL to obtain 332L of filtrate with a potency of 6620u / ml and a yield of 96.85%.

[0041] According to the volume ratio of 16%PEG2000 and filtrate washing solution 1:4, take 83L prepared 18%K 2 HPO 4 and 16% PEG2000 solution, stirred and mixed, and stood for 10 minutes to form a two-phase system. The above-mentioned filtrate was added to the two-phase system with stirring, and the pH was adjusted to 9.5-10.0 with liquid alkali, stirred for 15 minutes, and then statically Set aside for 40 minutes, layered, the volume of the upper layer is 82L, the upper layer and the lower lay...

Embodiment 3

[0047] Take 300L of lincomycin fermentation broth, the titer of the fermentation broth is 7730u / ml, add 5.8kg of oxalic acid under stirring, adjust the pH to 2.5~3.5, and add 0.36kg of yellow blood salt and 0.36kg of zinc sulfate in sequence at intervals of 20 minutes, continue Stir for 30 minutes, filter with plate and frame, and wash with oxalic acid or PH2.5-3.5 aqueous solution prepared with 2mol / L HCl to obtain 340L of filtrate, with a potency of 6550u / ml and a yield of 96.03%.

[0048] According to the volume ratio of 16%PEG2000 and filtrate washing solution 1:5, take 68L prepared 18%K 2 HPO 4 and 16% PEG2000 solution, stirred and mixed, and stood still for 10 minutes to form a two-phase system. The above-mentioned filtrate was added to the two-phase system with stirring, and the pH was adjusted to 9.5-10.0 with liquid alkali, stirred for 15 minutes, and left to stand. Place for 40 minutes, separate layers, the volume of the upper layer is 68L, and the upper and lower l...

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PUM

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Abstract

The invention relates to a lincomycin hydrochloride extraction method which comprises the following process steps: performing acidification pre-treatment on lincomycin fermentation broth, dual aqueous phase extraction, butanol extraction, hydrochloric acid reextraction, activated carbon decoloring, acetone crystallization, and drying, thereby obtaining a solid product lincomycin hydrochloride. Due to the adopted dual aqueous phase extraction technique for extract lincomycin hydrochloride, lincomycin can be effectively enriched, the use amount of butanol can be reduced, the solvent consumption can be reduced, dual aqueous phase raw materials used in the method are free of environment pollution, lincomycin can be produced in a large scale, economic and environment-friendly requirements can be met, and positive contribution can be made.

Description

technical field [0001] The invention belongs to the technical field of antibiotic extraction, and in particular relates to a method for extracting lincomycin hydrochloride by utilizing a two-phase extraction technique. Background technique [0002] Lincomycin Hydrochloride, also known as lincomycin or lincomycin, is a lincosamine-type alkaline antibiotic produced by the fermentation of Streptomyces Lincoensis. It can be used as an intermediate in medicine, veterinary medicine, feed additive and synthesis of clindamycin and its derivatives. [0003] At present, there are many methods for extracting lincomycin hydrochloride recorded in the literature, such as butanol (octyl alcohol) extraction-hydrochloric acid back-extraction technology, macroporous resin adsorption-butanol analysis-hydrochloric acid back-extraction technology, etc., wherein butanol (octyl alcohol) Alcohol) extraction-hydrochloric acid back-extraction technology has a large amount of solvent consumption, the...

Claims

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Application Information

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IPC IPC(8): C07H15/16C07H1/06
Inventor 裴立忠
Owner NINGXIA TAIYICIN BIOTECH CO LTD
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