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Applications of linoleoylethanolamine in improvement of gray mold resistance and bacterial leaf spot resistance of plants

A technology for linoleoyl ethanolamine and plant ash, which is applied in the directions of botanical equipment and methods, applications, plant growth regulators, etc., to achieve the effects of reducing dosage, enhancing resistance, and preventing and controlling botrytis and bacterial leaf spot

Active Publication Date: 2016-01-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and application of preparations for bacterial and fungal diseases with induced disease resistance are still relatively blank

Method used

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  • Applications of linoleoylethanolamine in improvement of gray mold resistance and bacterial leaf spot resistance of plants
  • Applications of linoleoylethanolamine in improvement of gray mold resistance and bacterial leaf spot resistance of plants
  • Applications of linoleoylethanolamine in improvement of gray mold resistance and bacterial leaf spot resistance of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Measure 51.8mL of 50mg / mL ethanol solution of linoleoylethanolamine NAE (18:2) (the drug was purchased from SIGMA); then slowly add 123.2mL of water, mix the mixed solution evenly, and finally add 25mL of organosilicon solution, after stirring evenly, the formulation was obtained.

[0046] Get 20 milliliters of the preparation of the present invention, add 10 liters of clear water and mix evenly, evenly spray on the surface of tomato blades whose seedling age is four leaves and one heart in the evening, until the leaves are wet, continue to spray for 2 days, use the tomato plants sprayed with clear water as control.

[0047] Randomly take tomato leaf samples sprayed with preparations and controls, use Trizol and Superscript II kits produced by Invitrogen Corporation of the United States to carry out RNA extraction, purification and reverse transcription of cDNA, and then use the StepONEPlusReal-TimePCRSystem instrument produced by Applied Biosystems Corporation of the U...

Embodiment 2

[0052] Measure 129.4 mL of 50 mg / mL ethanol solution of linoleoylethanolamine NAE (18:2) (the drug was purchased from SIGMA); slowly add 40.6 mL of water, mix the mixed solution evenly, and finally add 30mL of organosilicon solution was stirred evenly to obtain the preparation.

[0053] Get 15 milliliters of the preparation of the present invention, add 10 liters of clear water and mix evenly, evenly spray on the surface of tomato blades whose seedling age is five leaves and one heart in the evening, until the leaves are wet, continue to spray for 2 days, use the tomato plants sprayed with clear water as control.

[0054] Spray and inoculate the single Botrytis cinerea or bacterial leaf spot pathogen on the plants treated with the preparation and the control, so that the pathogen suspension is evenly distributed on the leaves. The inoculation concentration of Botrytis cinerea pathogen was 2×10 5 Spores / ml, the inoculum concentration for bacterial leaf spot was 1 × 10 7 Colo...

Embodiment 3

[0057] Measure 32.4 mL of 50 mg / mL ethanol solution of linoleoylethanolamine NAE (18:2) (the drug was purchased from SIGMA); slowly add 147.6 mL of water, mix the mixed solution evenly, and finally add 20mL of organosilicon solution was stirred evenly to obtain the preparation.

[0058] Get 10 milliliters of the preparation of the present invention, add 10 liters of clear water and mix evenly, evenly spray on the surface of tomato blades whose seedling age is three leaves and one heart in the evening, until the leaves are wet, continue to spray for 2 days, use the tomato plants sprayed with clear water as control.

[0059] Botrytis cinerea and bacterial leaf spot pathogens were sprayed and inoculated simultaneously on the plants treated with the preparation and the control, so that the suspension of the pathogens was evenly distributed on the leaves. The inoculation concentration of Botrytis cinerea pathogen was 2×10 5 Spores / ml, the inoculum concentration for bacterial leaf...

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Abstract

The present invention discloses applications of linoleoylethanolamine in improvement of gray mold resistance and bacterial leaf spot resistance of plants, and in preparation of plant gray mold and / or bacterial leaf spot resistance improving preparations. According to the present invention, linoleoylethanolamine is adopted as a main active ingredient to prepare the preparation, and the signal paths of jasmonic acid, salicylic acid and ethylene in plants are induced so as to significantly enhance the gray mold resistance and the bacterial leaf spot resistance of the plant, and reduce the economic loss of the gray mold and the bacterial leaf spot on the plant; and with the application of the preparation to prevent and control the gray mold and the bacterial leaf spot, the advantages of simpleness, easy performing and low cost are provided, the growth of the single or compound pathogenic bacteria of Botrytis cinerea and Pseudomonas syringae on the leaves and the plant disease diffusion can be significantly delayed and inhibited, and the gray mold resistance and the bacterial leaf spot resistance of the plant are substantially improved.

Description

technical field [0001] The invention relates to the field of phytochemical protection, in particular to the application of linoleoylethanolamine in improving plant resistance to botrytis cinerea and bacterial leaf spot. Background technique [0002] Under the background of climate change, the agricultural planting system is changing, the area of ​​occurrence of crop diseases is gradually increasing, the degree of damage is intensifying, and various fungal, bacterial and viral diseases are more rampant, which greatly weakens the impact of agricultural production. economic value, threatening the safety of agricultural production in the country. [0003] Studies have shown that in horticultural production, fungal diseases are the most various diseases among the known diseases, which can harm vegetables, fruit trees, flowers and other plants, accounting for about 80%-90% of the disease types, and have the most symptom types. Occurs in various parts of the plant. [0004] Curre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/20A01P3/00A01P1/00
Inventor 师恺喻景权邵淑君史军营
Owner ZHEJIANG UNIV
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