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Diagnosis method for promoting sludge uncoupling effect through uncoupling agent

A technology of uncoupling agent and diagnostic method, which is applied in the field of diagnosis of uncoupling effect, achieves good use value and application prospect, and the effect of simple and accurate determination method

Inactive Publication Date: 2016-01-06
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the technical problem that the existing diagnosis method cannot directly express the uncoupling effect in microorganisms, and to provide a diagnostic method for promoting the uncoupling effect of sludge by an uncoupling agent

Method used

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  • Diagnosis method for promoting sludge uncoupling effect through uncoupling agent
  • Diagnosis method for promoting sludge uncoupling effect through uncoupling agent

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Experimental program
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specific Embodiment approach 1

[0020] Specific embodiment 1: The diagnosis method of the decoupling agent promoting sludge decoupling effect in this embodiment is as follows:

[0021] 1. Take 30-50ml of sludge mixture from the activated sludge reaction system as the solution to be tested;

[0022] 2. Mix the solution to be tested in step 1 evenly, get 5ml of the solution to be tested and add 5ml of the trichloroacetic acid solution whose mass concentration is 5g / L;

[0023] 3. The solution obtained in step 2 was ultrasonically treated with an ultrasonic homogenizer for 3 minutes to obtain a homogeneous mixed solution. The mixed solution during the ultrasonic treatment was placed in a water tank and kept at a temperature of 15-25°C;

[0024] 4. Take 0.5ml homogeneous mixture and add 4.5ml 1×TAE buffer solution to mix;

[0025] 5. Use a 0.2 μm microporous filter to filter the mixed solution obtained in step 4 to remove solid matter;

[0026] Six, adopt fluorescence detector to measure the filtrate after fil...

specific Embodiment approach 2

[0036] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that in step 1, 31-35 ml of sludge mixed liquid is taken from the activated sludge reaction system as the solution to be tested. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0037] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 1, 33ml of sludge mixed solution is taken from the activated sludge reaction system as the solution to be tested. Others are the same as those in the first or second embodiment.

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Abstract

The invention provides a diagnosis method for promoting the sludge uncoupling effect through an uncoupling agent, and relates to a diagnosis method for the uncoupling effect. The method aims to solve the technical problem that diagnosis achieved through an existing method can not directly represent the uncoupling effect in microorganisms. The method includes the following steps that firstly, part of sludge mixed liquid is uniformly mixed, a trichloroacetic acid solution is added for ultrasonic processing, and homogeneous mixed liquid is obtained; the homogeneous mixed liquid is obtained and added into a buffering solution to be mixed; filtering is carried out; the fluorescence intensity of filtered liquid is measured; sludge concentration of a solution to be tested is measured; the sludge concentration is divided by the fluorescence intensity to obtain the specific ATP concentration; the sludge uncoupling effect is diagnosed according to the specific ATP concentration, when the specific ATP concentration is smaller than 60 RLU.L / mg, ATP synthesis in sludge is obviously inhibited, and metabolic uncoupling is generated. The method can be suitable for judging the effects, for promoting uncoupling in the sludge, of different uncoupling agents, and the test method is simple and accurate. The method belongs to the field of uncoupling effect diagnosis of the uncoupling agents.

Description

technical field [0001] The invention relates to a diagnostic method for uncoupling effect, in particular to a detection method for measuring the specific ATP concentration of activated sludge by using a SystemSureplus ATP fluorescence detector, and indirectly expressing the catabolism and metabolism of microorganisms in activated sludge by measuring the specific ATP concentration A method for the degree of anabolic uncoupling. Background technique [0002] At present, the main treatment process adopted by China's sewage treatment plants is the activated sludge process, in which the activated sludge is mainly composed of microbial groups and their attached organic and inorganic substances. Microbial groups mainly include bacteria, protozoa and algae. This process mainly uses microorganisms in sludge to decompose organic nutrients in water to achieve the purpose of sewage treatment, and the large amount of excess activated sludge produced by this process has become the main b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 郭婉茜冯骁驰任南琪郑禾山
Owner HARBIN INST OF TECH
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